Verification of barley seed dormancy loci via linked molecular markers
Verification of barley seed dormancy loci via linked molecular markers
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DOI：
10.1007/bf00222956
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发表时间：
1996-01-01
影响因子：
5.4
通讯作者：
Romagosa, I
中科院分区：
文献类型：
--
作者：
Han, F;Ullrich, SE;Romagosa, I
大麦种子休眠性是一个相对复杂的性状。先前通过数量性状基因座分析鉴定了几个休眠基因座。本研究通过连锁分子标记在携带特定休眠等位基因的亲本之间进行了三次正反交，以验证个体休眠位点效应和潜在表达。F-2后代的分析表明，在由标记Ale和ABC 302在7号染色体长臂两侧的基因座的休眠等位基因有一个主要的影响休眠，并至少部分上位的休眠基因座在ABC 309-MWG 851区间附近的7号染色体长臂的端粒。在Ale-ABC 302区间中不存在休眠等位基因的情况下，ABC 309-MWG 851区间中的等位基因对休眠产生中等至大的影响。细胞质对休眠的影响也被观察到。相对较高水平的休眠的后代的发芽率比非休眠或少休眠的后代，更易变，显然是由于环境的影响。在Ale-ABC 302区间中去除休眠等位基因，或在ABC 309-MWG 851区间中引入休眠等位基因，应足以在育种程序中调整休眠水平以适应各种生产情况和最终用途。通过连锁分子标记验证休眠位点允许在应用育种程序中操纵这些位点。
Seed dormancy is a relatively complex trait in barley (Hordeum vulgare L.). Several dormancy loci were identified previously by quantitative trait locus analysis. Three reciprocal crosses were made in the present study between parents carrying specific dormancy alleles via linked molecular markers to verify individual dormancy locus effects and potential expression. Analyses of F-2 progenies revealed that the dormancy allele at the locus flanked by the markers Ale and ABC302 on the long arm of chromosome 7 had a major effect on dormancy, and was at least partly epistatic to the dormancy locus in the ABC309-MWG851 interval near the telomere of the long arm of chromosome 7. In the absence of the dormancy allele in the Ale-ABC302 interval, the allele in the ABC309-MWG851 interval exerted moderate to large effects on dormancy. Cytoplasmic effects on dormancy were also observed. The germination percentages of progeny with relatively high levels of dormancy were more variable than those of non-dormant or less-dormant progeny, apparently due to environmental effects. Removal of the dormancy allele in the Ale-ABC302 interval, or introducing the dormancy allele in the ABC309-MWG851 interval, should suffice for adjusting dormancy levels in breeding programs to suit various production situations and end uses. The verification of dormancy loci via linked molecular markers allows manipulation of these loci in applied breeding programs.