Exercise decreases plasma antioxidant capacity and increases urinary isoprostanes of IPF patients
Exercise decreases plasma antioxidant capacity and increases urinary isoprostanes of IPF patients
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DOI：
10.1016/j.rmed.2010.07.021
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发表时间：
2010-12-01
影响因子：
4.3
通讯作者：
Gomez-Marin, O.
中科院分区：
文献类型：
--
作者：
Jackson, R.;Ramos, C.;Gomez-Marin, O.
我们在29例典型的IPF患者中检测了是否可检测到全身氧化应激标志物，以及这些标志物在低水平运动后是否增加。（NT proBNP），以及血浆和尿液样品中的异前列腺素和总亚硝酸盐。测定受试者C在50 W半卧自行车上运动至呼吸困难时尿中H_2O_2的含量，并在运动后立即取血测定相同的变量，同时取6名40岁以上的正常人安静时的血浆和尿样品，以建立比较NT proBNP、亚硝酸盐、H_2O_2和TAC测定值静息时和运动后血浆NT proBNP均升高，提示肺动脉高压患者静息时NT proBNP浓度明显高于正常对照组，IPF患者静息时血浆异前列腺素水平高于正常人，运动后IPF患者尿异前列腺素水平显著升高（经符号秩和检验P = 0 0 47），运动后血浆总抗氧化能力显著降低（符号等级检验P <0001）运动后血浆和尿亚硝酸盐均无显著变化，部分IPF患者运动后尿H2O2浓度相当高显示静息时的全身氧化应激，可检测为循环中异前列腺素的增加运动后尿异前列腺素的增加和血浆TAC的减少表明，IPF患者在低水平运动期间产生活性氧（ROS）。
We tested whether markers of systemic oxidant stress were detectable in 29 typical IPF patients, and whether these increased after low level exercise We obtained resting plasma for measurement of amino terminal pro brain natriuretic peptide (NT proBNP), and plasma and urine samples for isoprostanes and total nitrite Total antioxidant capacity (TAC) was measured in plasma, and H2O2 was measured in urine Subject c exercised at 50 W on a semi recumbent bicycle until limited by dyspnea Samples were obtained immediately after exercise for measurement of the same variablesPlasma and urine samples were also obtained at rest from 6 normal individuals over 40 years of age solely to establish comparison values for NT proBNP, nitrite, H2O2 and TAC assaysPlasma NT proBNP was high at rest and after exercise, suggesting pulmonary arterial hypertension IPF patients' resting NT proBNP concentrations apparently exceeded those of normal controls IPF plasma isoprostanes at rest exceeded the normals IPF urine isoprostanes increased significantly after exercise (P = 0 047 by signed lank test), and, plasma TAC decreased significantly after exercise (P < 0 001 by signed rank test) Neither plasma nor urine nitrite changed significantly after exercise H2O2 concentration was quite high after exercise in some IPF subjects urineIPF patients demonstrate systemic oxidant stress at rest detectable as increased isoprostanes in the circulation An increase in urine isoprostanes and a decrease in plasma TAC after exercise suggest that reactive oxygen species (ROS) are produced during low level exercise done by IPF patients Published by Elsevier Ltd