Protocol for detection of in vitro R-loop formation using dot blots.
Protocol for detection of in vitro R-loop formation using dot blots.
复制标题
使用斑点印迹检测体外 R 环形成的方案。
DOI：
10.1016/j.xpro.2024.102857
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Forero,Adriana
中科院分区：
文献类型：
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作者：
Dowling,JackW;Smith,JulianR;Forero,Adriana
斑点印迹分析是一种允许快速和方便地检测和鉴定核酸和蛋白质的技术。在这里，我们提供了一个指导核酸分离从真核细胞和样品处理，以检测RNA/DNA杂交。然后，我们提供了量化点信号强度的详细步骤。该方案可适用于导致R环积累的筛选条件。有关该方案使用和执行的完整详细信息，请参见Smith等人1
Dot-blot analysis is a technique that allows for fast and convenient detection and identification of nucleic acids and proteins. Here, we provide a guide for nucleic acid isolation from eukaryotic cells and sample processing to detect RNA/DNA hybrids. We then provide detailed steps to quantify dot signal intensity. This protocol can be adapted for screening conditions that result in the accumulation of R-loops.For complete details on the use and execution of this protocol, please refer to Smith et al.1