Design and fabrication of an integrated cell processor for single embryo cell manipulation
Design and fabrication of an integrated cell processor for single embryo cell manipulation
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DOI：
10.1039/b404990j
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Park, JO
中科院分区：
文献类型：
--
作者：
Park, J;Jung, SH;Park, JO
本文介绍了一种用于单个胚胎细胞自动处理的集成细胞处理器。集成处理器可以执行各种功能，例如细胞运输、分离、定向和固定。这些功能是不可或缺的，经常用于单细胞的操作，但只能由熟练的操作员执行。本研究的目的是使用MEMS方法集成和自动化这些功能，以实现有效的细胞操作。在细胞被运输到其中的微通道中使用聚吡咯（PPy）阀进行细胞的分离。通过电旋转（ER）控制细胞的取向，并通过从微孔中抽吸来固定靶细胞。所有这些功能都在一个芯片上无缝实现。小鼠（B6CBA）胚胎细胞的实验结果表明，该装置可以取代常规和繁琐的手工工作。预计集成芯片将大大有助于更快、更可靠地操纵细胞。
This paper presents an integrated cell processor for the automatic handling of individual embryo cells. The integrated processor can perform various functions such as cell transport, isolation, orientation, and immobilization. These functions are indispensable and frequently used for the manipulation of single cells, but can only be carried out by a skillful operator. The purpose of this study was the integration and automation of these functions for effective cell manipulation, using a MEMS approach. The isolation of a cell was performed using polypyrrole (PPy) valves in a microchannel into which cells were transported. The orientation of cells was controlled by electrorotation (ER), and the target cell was immobilized by suction from a microhole. All of these functions were seamlessly realized on a single chip. Excellent experimental results with mouse (B6CBA) embryo cells showed that this device could substitute for routine and cumbersome manual work. It is expected that the integrated chip will contribute significantly to faster and more reliable manipulation of cells.