Ectopic expression of OsMADS1 caused dwarfism and spikelet alteration in rice
Ectopic expression of OsMADS1 caused dwarfism and spikelet alteration in rice
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OsMADS1 的异位表达导致水稻矮化和小穗改变
DOI：
10.1007/s10725-016-0220-9
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发表时间：
2017-04-01
影响因子：
4.2
通讯作者：
Li, Yun-Feng
中科院分区：
文献类型：
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作者：
Wang, Ling;Zeng, Xiao-Qin;Li, Yun-Feng
在水稻中，e类基因OsMADS1的作用是确定外稃和古稃的身份。本研究将带有CaMV35S启动子的OsMADS1基因转化到粳稻品种中华11号中。所有转基因植株均成功表现出相似的表型，包括矮化、穗形扭曲、分枝和小穗数量减少以及不育外稃拉长。组织学分析表明，外稃发育有硅化的表皮细胞和厚壁细胞，这是野生型外稃所缺乏的，表明外稃具有外稃或古外稃的特征。对部分标记基因在外稃、古外稃和不育外稃中的表达分布进行了详细分析。下垂叶（DL）基因和OsMADS6基因分别只在正常外稃和古茎中表达。在伸长的不育外稃中，检测到DL基因的高表达，而OsMADS6基因未表达，说明不育外稃转化为外稃而未转化为原稃。这些结果为阐明水稻外稃向不育外稃的演化机制提供了线索。qPCR分析还表明，OsMADS1的异位表达诱导了油菜素内酯和赤霉素酸的异常激活，从而导致茎和穗发育缺陷。
In rice, an E-class gene, OsMADS1, acts to specify the identities of the lemma and palea. In this study, the OsMADS1 gene with a CaMV35S promoter was transformed into a japonica cultivar, Zhonghua 11. All transgenic plants successfully showed similar phenotypes, including dwarfism, distorted panicles, decreased numbers of branches and spikelets, and elongated sterile lemma. Histological analysis showed that the elongated sterile lemma developed with silicified epidermal and sclerenchymal cells, which were lacking in the wild-type sterile lemma, suggesting that the elongated sterile lemma had assumed the identity of the lemma or palea. Some marker genes were subjected to a detailed analysis of the distribution of their expression among the lemma, palea and sterile lemma. DROOPING LEAF (DL) and OsMADS6 genes were only expressed in the normal lemma or palea, respectively. In the elongated sterile lemma, a high level of DL gene expression was detected, while no expression of OsMADS6 was found, implying that the sterile lemma transformed into the lemma but not the palea. These results provide clues to elucidate the mechanism of evolution from lemma to sterile lemma in rice. qPCR analysis also suggested that the ectopic expression of OsMADS1 induced abnormal brassinosteroid and gibberellin acid activation, and then resulted in developmental defects in the stem and panicle.