High-Frequency Pulsed Low-Level Diode Laser Therapy Accelerates Wound Healing of Tooth Extraction Socket: An In Vivo Study
High-Frequency Pulsed Low-Level Diode Laser Therapy Accelerates Wound Healing of Tooth Extraction Socket: An In Vivo Study
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DOI：
10.1002/lsm.22560
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发表时间：
2016-12-01
影响因子：
2.4
通讯作者：
Izumi, Yuichi
中科院分区：
文献类型：
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作者：
Noda, Masahiro;Aoki, Akira;Izumi, Yuichi
背景和目的：本研究旨在评估高频脉冲（HiFP）低强度激光治疗（LLLT）对大鼠拔牙窝早期伤口愈合的影响。研究设计/材料和方法：从6周龄的Sprague-Dawley大鼠中提取双侧上颌第一磨牙。通过HiFP低水平二极管激光照射（904-910 nm）处理右侧的窝;左侧作为未照射对照。提取后立即使用LLLT（0.28 W，30 kHz，200 ns脉冲，0.6%占空比，61.2 J/cm（2）总功率密度），此后每24小时使用一次。拔牙后3或7天切除包括牙槽窝在内的上颌骨。在第0、3和7天评价软组织愈合。通过显微计算机断层扫描评估拔牙窝的骨矿物质含量（BMC）、骨体积（BV）和骨密度（BMD），并在第7天进行组织形态计量学分析。第3天进行成骨标志物表达的实时荧光定量PCR分析和增殖细胞核抗原（PCNA）阳性细胞的免疫组化检测。与对照部位相比，照射后拔牙部位的未上皮化面积显著减少（P = 0.04），激光治疗部位的BMC、BV和BMD显著增加（P分别为0.004、0.006和0.009）。第7天，激光治疗部位新形成的未成熟编织骨的平均高度较高（P = 0.24）。结论：低强度半导体激光照射能促进拔牙窝软硬组织的愈合。(C)2016 Wiley Periodicals，Inc.
Background and Objective: This study aimed to evaluate the effects of high-frequency pulsed (HiFP) low-level laser therapy (LLLT) on early wound healing of tooth extraction sockets in rats.Study Design/Materials and Methods: Bilateral maxillary first molars were extracted from 6-week-old Sprague-Dawley rats. Sockets on the right were treated by HiFP low-level diode laser irradiation (904-910 nm); the left sides served as unirradiated controls. LLLT (0.28 W, 30kHz, 200-ns pulse, 0.6% duty cycle, 61.2 J/cm(2) total power density) was employed immediately after extraction and every 24 hours thereafter. The maxillae including the sockets were resected 3 or 7 days after extraction. Soft-tissue healing was evaluated on days 0, 3, and 7. The bone mineral content (BMC), bone volume (BV), and bone mineral density (BMD) of the extraction sockets were evaluated by microcomputed tomography, and histomorphometric analysis was carried out on day 7. Real-time PCR analysis of osteogenic marker expression and immunohistochemical detection of proliferating cell nuclear antigen (PCNA)-positive cells were performed on day 3.Results: Compared with control sites, the un-epithelialized areas of the extracted sites were significantly reduced by irradiation (P = 0.04), and the BMC, BV, and BMD of laser-treated sites were significantly increased (P = 0.004, 0.006, and 0.009, respectively). On day 7, the mean height of newly formed immature woven bone was higher in laser-treated sites (P = 0.24). On day 3, laser-treated sites showed significantly higher osteocalcin mRNA expression (P = 0.04) and PCNA-positive cell numbers (P = 0.01).Conclusion: HiFP low-level diode laser irradiation enhanced soft-and hard-tissue healing of tooth extraction sockets. (C) 2016 Wiley Periodicals, Inc.