The construction of gene knockouts in the cyanobacterium Synechocystis sp. PCC 6803.
The construction of gene knockouts in the cyanobacterium Synechocystis sp. PCC 6803.
复制标题
蓝藻集胞藻属基因敲除的构建。
DOI：
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发表时间：
2004
影响因子：
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通讯作者：
J. Eaton‐Rye
中科院分区：
文献类型：
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作者：
J. Eaton‐Rye
提出了一系列用于集胞藻 PCC 6803 野生型 (wt) 和突变株的储存、生长、转化和表征的方案。这些方案包括基因组 DNA 的分离以及在该生物体中构建特定基因敲除所需的策略。这种蓝藻已被广泛用作光合作用研究的模型，其基因组序列以及已构建的突变体数据库可在 CyanoBase (http://kazusa.or.jp/cyano/) 上获取。本章提供的详细信息并不假设任何先前使用蓝藻的经验，旨在使新研究人员能够利用已适用于该系统的广泛基因修饰和突变图谱技术。
A series of protocols are presented for the storage, growth, transformation, and characterization of wild-type (wt) and mutant strains of Synechocystis PCC 6803. These protocols include the isolation of genomic DNA and the strategies required for the construction of specific gene knockouts in this organism. This cyanobacterium has been used widely as a model for photosynthesis research and the sequence of its genome, together with a database of mutants that have already been constructed, is available at CyanoBase (http://kazusa.or.jp/cyano/). The details provided in this chapter do not assume any previous experience in working with cyanobacteria and are intended to enable new investigators to take advantage of a wide-range of gene modification and mutation mapping techniques that have been adapted for use in this system.
基因修饰和突变图谱研究光系统 II 的功能。
影响因子：
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作者：
Vermaas,WF
通讯作者：
Vermaas,WF