NMDA receptor blockade prevents LTD, but not LTP induction by intracellular tetanization
NMDA receptor blockade prevents LTD, but not LTP induction by intracellular tetanization
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DOI：
10.1097/00001756-199912160-00027
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发表时间：
1999-12-16
期刊：
影响因子：
1.7
通讯作者：
Volgushev, M
中科院分区：
文献类型：
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作者：
Chistiakova, M;Balaban, P;Volgushev, M
将细胞内强直化作用（在没有伴随的突触前刺激的情况下激活突触后细胞）应用于大鼠视皮层切片中的第II/III层锥体细胞。在标准的细胞外介质中，细胞内强直导致LTP（21的43输入）或LTD（14的43输入），幅度变化的方向取决于初始成对脉冲易化（PPF）的比率：输入与高初始PPF比率通常是增强，和输入与初始低PPF最经常被压抑或没有变化。当施加在NMDA受体（50 μ M APV）的封锁，细胞内强直未能诱导LTD，但仍然能够诱导LTP（14 26输入）。虽然LTP可以发生在输入，低和高的初始PPF比率，幅度变化和初始PPF比率之间的相关性仍然存在：增强更强的输入与最初较高的PPF。这些数据表明，细胞内强直同时激活NMDA受体依赖的LTD机制和NMDA受体非依赖的LTP机制，突触增益的最终变化取决于它们的平衡。(C)1999年利平科特威廉姆斯&威尔金斯。
INTRACELLULAR tetanization, the activation of a postsynaptic cell without concomitant presynaptic stimulation, was applied to layer II/III pyramidal cells in slices of rat visual cortex. In standard extracellular medium, intracellular tetanization led to LTP (21 of 43 inputs) or LTD (14 of 43 inputs), the direction of the amplitude change depending on initial paired-pulse facilitation (PPF) ratio: inputs with high initial PPF ratio were usually potentiated, and inputs with initially low PPF were most often depressed or did not change. When applied during blockade of NMDA receptors (50 mu M APV), intracellular tetanization failed to induce LTD, but was still capable of inducing LTP (14 of 26 inputs). Although LTP could occur in inputs with both, low and high initial PPF ratio, the correlation between the amplitude change and initial PPF ratio remained: potentiation was stronger in inputs with initially higher PPF. These data suggest that intracellular tetanization activated simultaneously NMDA receptor-dependent LTD mechanisms and NMDA receptor-independent LTP mechanisms, the final change of synaptic gain depending on their balance. (C) 1999 Lippincott Williams & Wilkins.