EFFECT OF CITRATE ON ACTIVITIES OF 6-PHOSPHOFRUCTOKINASE FROM NERVOUS AND MUSCLE TISSUES FROM DIFFERENT ANIMALS AND ITS RELATIONSHIP TO REGULATION OF GLYCOLYSIS
EFFECT OF CITRATE ON ACTIVITIES OF 6-PHOSPHOFRUCTOKINASE FROM NERVOUS AND MUSCLE TISSUES FROM DIFFERENT ANIMALS AND ITS RELATIONSHIP TO REGULATION OF GLYCOLYSIS
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DOI：
10.1042/bj1660123
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发表时间：
1977-01-01
影响因子：
4.1
通讯作者：
WILLIAMS, T
中科院分区：
文献类型：
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作者：
NEWSHOLME, EA;SUGDEN, PH;WILLIAMS, T
柠檬酸盐可抑制除昆虫外的动物王国中不同动物的肌肉和神经组织中磷酸果糖激酶[EC2.7.1.11]的活性。研究了9种不同昆虫飞行肌和蝗虫脑神经节的酶：未观察到柠檬酸盐抑制。来自两种需氧（例如，鸽子的胸肌）和厌氧（例如，鱼的肌肉、猎鸟的胸肌）肌肉。这表明，这种抑制在降低动物在饥饿和/或长时间运动时骨骼肌中葡萄糖利用率方面具有重要的生理意义。酮体能显著降低蛙缝匠肌和腓肠肌对葡萄糖的利用率。后者提高了G-6-P和柠檬酸盐含量的腓肠肌，表明磷酸果糖激酶的柠檬酸盐抑制可能是，在一定程度上，负责糖酵解速率下降。这些发现提供了证据表明，葡萄糖-脂肪酸-酮体循环的概念涉及有氧和无氧骨骼肌和神经组织，除了昆虫。在后者中，周期的概念可能不适用。
Citrate inhibits the activities of phosphofructokinase [EC 2.7.1.11] from muscles and nervous tissues from different animals across the Animal Kingdom except for the insects. The enzymes from the flight muscle of 9 different insects and the cerebral ganglion of the locust were investigated: no inhibition by citrate was observed. Inhibition was observed with the enzymes from both aerobic (e.g., pectoral muscle of pigeon) and anaerobic (e.g., fish muscle, pectoral muscle of the game birds) muscles. It is suggested that this inhibition is of physiological importance in decreasing the rate of glucose utilization in skeletal muscle of animals during starvation and/or prolonged exercise. The rates of glucose utilization by the sartorius and gastrocnemius muscles of the frog were markedly decreased by ketone bodies. The latter elevated the G-6-P and citrate contents of the gastrocnemius muscle, indicating that citrate inhibition of phosphofructokinase could be, in part, responsible for the decreased rate of glycolysis. These findings provide evidence that the concept of the glucose-fatty acid-ketone-body cycle involves aerobic and anaerobic skeletal muscle and nervous tissue from a wide range of animals except the insects. In the latter the concept of the cycle may not be applicable.