Improved mRNA electroporation method for Xenopus neurula embryos
Improved mRNA electroporation method for Xenopus neurula embryos
复制标题
非洲爪蟾神经胚胚胎 mRNA 电穿孔方法的改进
DOI：
10.1002/gene.10094
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发表时间：
2002
期刊：
影响因子：
1.5
通讯作者：
K. Takeshima
中科院分区：
文献类型：
--
作者：
S. Sasagawa;T. Takabatake;Y. Takabatake;T. Muramatsu;K. Takeshima
摘要：电穿孔技术为分析鸡胚胎系统的基因调控提供了新的途径。然而，将这种方法应用于另一种胚胎学模式生物非洲爪哇，仍有许多困难需要克服。特殊设计的电极，表达的荧光素酶活性的检测，以及绿色荧光蛋白的直接显示，使我们能够优化爪哇胚胎的电穿孔条件。与使用DNA相比，使用mRNA的表达效率提高了120倍，而且这种效果在电穿孔后立即显现。与针型电极相比，本文采用的非接触式电穿孔对细胞和组织的损伤更小，适用于有效地应用于早期胚胎。此外，mRNA电穿孔技术也适用于其他DNA电穿孔效果不明显的系统，因为其表达效率较低。创世纪33：81-85,2002。©2002 Wiley-Liss公司
Summary: Electroporation has led to new approaches to the analysis of gene regulation of the chick embryonic system. However, application of this method to Xenopus, another model organism of embryology, has left many difficulties to be overcome. The specially devised electrodes, the examination of luciferase activities expressed, and the direct visualization of green fluorescence protein allow us to optimize the conditions of electroporation for Xenopusembryos. The use of mRNA rather than DNA improved the expression efficiency 120 times more than for the case of plasmid DNA, and the effect emerged more immediately after electroporation. The noncontact electroporation adopted here caused less damage to cells and tissues than with the needle type electrode, making it practical for efficient application to early embryos. Furthermore, the mRNA electroporation technique is applicable for other systems in which the DNA electroporation has not had any significant effect because of its low expression efficiency. genesis 33:81–85, 2002. © 2002 Wiley‐Liss, Inc.
影响因子：
3.4
作者：
Karikó, K;Kuo, A;Langer, DJ
通讯作者：
Langer, DJ
影响因子：
4.6
作者：
Robert Kos;M. Reedy;Randy L. Johnson;C. Erickson
通讯作者：
Robert Kos;M. Reedy;Randy L. Johnson;C. Erickson