Nanoparticle-mediated intravesical delivery of conditioned medium derived from mesenchymal stem cells for interstitial cystitis/bladder pain syndrome treatment
Nanoparticle-mediated intravesical delivery of conditioned medium derived from mesenchymal stem cells for interstitial cystitis/bladder pain syndrome treatment
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纳米颗粒介导的间质干细胞条件培养基膀胱内递送用于间质性膀胱炎/膀胱疼痛综合征的治疗
DOI：
10.1016/j.apmt.2021.101144
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发表时间：
2021-08-14
影响因子：
8.3
通讯作者：
Zhou, Xiangfu
中科院分区：
文献类型：
--
作者：
Lin, Zhijun;Liu, Bolong;Zhou, Xiangfu
间质性膀胱炎/膀胱疼痛综合征（IC/BPS）是一种以伴有下尿路症状的骨盆疼痛为特征的危重疾病。有效的治疗策略仍然具有挑战性，由于其难以捉摸的发病机制。间充质干细胞（MSC）疗法为IC/BPS的治疗提供了一种有前景的治疗方法。然而，干细胞治疗的伦理争议和致瘤风险限制了其临床应用。在此，我们开发了一种纳米颗粒，其封装来自干细胞的条件培养基（CM），并在表面上涂覆有阳离子N-三甲基壳聚糖氯化物（TMC），以提高IC/BPS膀胱内治疗中上皮屏障的穿透。TMC涂层通过促进抗炎作用和BDNF/TrkB信号传导，增强了载CM纳米粒在膀胱内的蓄积，从而提高了对机械阈值和排尿功能的治疗效果，为IC/BPS膀胱内治疗的经粘膜给药平台提供了巨大的应用潜力。(c)2021爱思唯尔有限公司保留所有权利。
Interstitial cystitis/bladder pain syndrome (IC/BPS) is a critical disease characterized by debilitating pelvic pain associated with lower urinary tract symptoms. Effective therapeutic strategies remain challenging due to their elusive pathogenesis. Mesenchymal stem cell (MSC) therapy offers a promising therapeu-tic approach for IC/BPS treatment. Yet, the ethical arguments and tumorigenic risks from cell therapy by stem cells restrict the clinical application. Herein, we developed a nanoparticle that encapsulates the conditioned medium (CM) from stem cells and coated with cationic N-trimethyl chitosan chloride (TMC) on the surface to improve the penetration of epithelial barrier in intravesical therapy for IC/BPS. The TMC coating enhanced the accumulation of CM-loaded nanoparticles in the urothelium and subsequently im -proved the therapeutic efficacy on the mechanical threshold and micturition function through facilitating the effect of anti-inflammation and BDNF/TrkB signaling, which provides a great potential application on the transmucosal delivery platform for intravesical therapy of IC/BPS. (c) 2021 Elsevier Ltd. All rights reserved.