HEPARIN AFFINITY - PURIFICATION OF A TUMOR-DERIVED CAPILLARY ENDOTHELIAL CELL-GROWTH FACTOR
HEPARIN AFFINITY - PURIFICATION OF A TUMOR-DERIVED CAPILLARY ENDOTHELIAL CELL-GROWTH FACTOR
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DOI：
10.1126/science.6199844
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
KLAGSBRUN, M
中科院分区：
文献类型：
--
作者：
SHING, Y;FOLKMAN, J;KLAGSBRUN, M
一种刺激[牛]毛细血管内皮细胞增殖的肿瘤[大鼠软骨肉瘤]衍生生长因子对肝素有很强的亲和力。这种肝素亲和性使得有可能在快速的2步程序中将生长因子纯化为单一条带制剂。纯化的生长因子是一种阳离子多肽，其分子量约为18，000，并且在约1 ng/ml的浓度下刺激毛细血管内皮细胞增殖。
A tumor [rat chondrosarcoma] derived growth factor that stimulates the proliferation of [bovine] capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid 2-step procedure. The purified growth factor is a cationic polypeptide, has a MW of .apprx. 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 ng/ml.