Inhibitory effects of dissolved organic matter on erythromycin bioavailability and possible mechanisms
Inhibitory effects of dissolved organic matter on erythromycin bioavailability and possible mechanisms
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溶解有机物对红霉素生物利用度的抑制作用及其可能机制
DOI：
10.1016/j.jhazmat.2019.04.073
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发表时间：
2019
影响因子：
13.6
通讯作者：
Wang Wei
中科院分区：
文献类型：
--
作者：
Liu Zhiping;Delgado Moreno Laura;Lu Zhijiang;Zhang Sufen;He Yan;Gu Xin;Chen Ziyuan;Ye Qingfu;Gan Jay;Wang Wei
大环内酯类抗生素广泛应用于水生环境中。与许多新出现的污染物不同，大环内酯在其胺基上带正电荷，并且可能与溶解的有机物（DOM）的负电荷基团相互作用，这可能会改变大环内酯的生物累积，但尚待探索。本文采用14 C示踪技术研究了不同DOM（LeHA、PPHA、SRHA和SRFA）对红霉素（一种重要的大环内酯类抗生素）在水生生物中富集的影响。结果表明，DOM显著抑制了有机体对ERY的吸收（P< 0.05）。在DOM浓度为20 mg L−1的情况下，斑马鱼和浮萍的平均平衡浓度（Ce）分别降低了28.1-40.6%和10.9-25.8%，相应的生物浓缩系数（BCF）分别降低了15.9-32.8%和10.9- 18.5%。SRHA和SRFA的抑制作用强于LeHA和PPHA，可能是由于它们的羧基含量较高。结果表明，DOM通过−COO−和ERY+的离子键合、氢键和疏水分配形成DOM-ERY复合物，抑制ERY的生物有效性。这项研究是第一次报告DOM对红霉素生物利用度的抑制作用，对更好地了解大环内酯类药物的风险具有重要意义。
Macrolides are widely used antibiotics with ubiquitous occurrence in aquatic environments. Unlike many emerging contaminants, macrolides are positively charged on their amine groups and are likely to interact with negative charge groups of dissolved organic matters (DOMs), which may alter macrolide bioaccumulation but yet to be explored. Here we evaluated the effects of different DOM (LeHA, PPHA, SRHA and SRFA) on erythromycin (an important macrolide) bioaccumulation into aquatic biota with14C tracing. Results showed that ERY uptake in organisms was significantly inhibited by DOM (P< 0.05). In the presence of DOM at 20 mg L−1, the averaged equilibrium concentration (Ce) decreased by 28.1–40.6% for zebrafish and 10.9–25.8% for duckweed, corresponding to reductions in the bioconcentration factor (BCF) by 15.9–32.8% and 10.9–18.5%, respectively. Likely due to their higher carboxyl group content, SRHA and SRFA exhibited stronger inhibitory effects than LeHA or PPHA. The possible interactions between ERY and DOM were explored and results suggested that DOM inhibited ERY bioavailability by forming DOM-ERY complexes via ionic bonding of −COO−and ERY+, hydrogen bonding and hydrophobic partitioning. This study was the first to report on inhibitory effects of DOM on erythromycin bioavailability and has important implications for better understanding risks of macrolides.