Immunohistochemical study of extracellular matrix components in epiretinal membranes of vitreoproliferative retinopathy and proliferative diabetic retinopathy
Immunohistochemical study of extracellular matrix components in epiretinal membranes of vitreoproliferative retinopathy and proliferative diabetic retinopathy
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DOI：
10.1177/112067210501500312
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发表时间：
2005-05-01
影响因子：
1.7
通讯作者：
Agnantis, NJ
中科院分区：
文献类型：
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作者：
Ioachim, E;Stefaniotou, M;Agnantis, NJ
目的。在正常和伤口愈合条件下，细胞的迁移、增殖、分化和粘附以及其他细胞功能受到周围细胞外基质的影响。视网膜前膜的形成是伤口愈合过程，是视网膜疾病的严重并发症，其中最重要的是增殖性糖尿病视网膜病变（PDR）和增殖性玻璃体视网膜病变（PVR）。在本研究中，作者研究了各种细胞外基质成分的表达，特别是腱蛋白、纤连蛋白、层粘连蛋白、IV型胶原蛋白和MMP-3糖蛋白，以及每种类型的上皮膜中神经胶质原纤维酸性蛋白的表达，以阐明这些分子在这两类膜形成中的作用。方法。作者使用针对上述细胞外基质成分的抗体，对 14 个 PVR 和 14 个 PDR 膜进行了免疫组织化学分析。腱蛋白和纤连蛋白被视为细胞外基质中的主要成分，而层粘连蛋白和 IV 型胶原蛋白被检测为两种类型膜中的次要成分。与 PDR 膜相比，PVR 膜中的纤连蛋白表达更高 (p=0.0035)。还观察到其表达与增殖活性（p=0.15）和IV型胶原蛋白表达（p<0.0001）呈正相关。 结果。腱蛋白表达与 PDR 膜中胶质纤维酸性蛋白阳性细胞呈正相关 (p=0.04)。在 PDR 膜中观察到位于血管周围的 IV 型胶原蛋白水平较高 (p=0.0031)。结论。结果表明，细胞外基质成分似乎参与了 PVR 和 PDR，有助于组织重塑，并且可能通过不同的致病途径，这可以反映这两类膜的不同发育阶段。
PURPOSE. The migration, proliferation, differentiation, and adhesion of cells and other cellular functions are influenced by the surrounding extracellular matrix in normal and wound healing conditions. The formation of epiretinal membranes, a wound healing process, is a serious complication of retinal diseases, the most important being proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR). In the present study, the authors investigated the expression of various extracellular matrix components and in particular tenascin, fibronectin, laminin, collagen IV, and MMP-3 glycoprotein as well as the expression of glial fibrillary acidic protein in each type of epithelial membrane in order to elucidate the role of these molecules in the formation of these two types of membranes.METHODS. The authors performed immunohistochemistry in 14 PVR and 14 PDR membranes, using antibodies against the above mentioned extracellular matrix components. Tenascin and fibronectin were observed as major components in the extracellular matrix, while laminin and collagen type IV were detected as minor components in both types of membranes. A higher fibronectin expression in PVR compared with PDR membranes was found (p=0.0035). A positive relationship of its expression with the proliferative activity (p=0.15) and collagen type IV expression (p < 0.0001) was also observed.RESULTS. Tenascin expression was positively correlated with glial fibrillary acidic protein positive cells in PDR membranes (p=0.04). Collagen type IV localized around vessels was observed with high levels in PDR membranes (p=0.0031).CONCLUSIONS. The results indicated that the extracellular matrix components seem to be involved in PVR and PDR, contributing to tissue remodeling and perhaps by different pathogenetic pathways, which could reflect different stages of development in these two types of membranes.