Targeted Microfluidic Manufacturing to Mimic Biological Microenvironments: Cell-Encapsulated Hollow Fibers
Targeted Microfluidic Manufacturing to Mimic Biological Microenvironments: Cell-Encapsulated Hollow Fibers
复制标题
模拟生物微环境的定向微流体制造：细胞封装的中空纤维
DOI：
10.1021/acsmacrolett.1c00159
复制
发表时间：
2021
影响因子：
7.015
通讯作者：
Hashemi, Nicole N.
中科院分区：
文献类型：
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作者：
McNamara, Marilyn C.;Aykar, Saurabh S.;Montazami, Reza;Hashemi, Nicole N.
目前，血脑屏障(BBB)对治疗广泛的中枢神经系统疾病构成了挑战；仍然需要可靠的BBB模型来了解和操纵分子进入大脑的转移，从而提高治疗的效率。在这项研究中，中空的、载细胞的微纤维被制造和研究，作为建立血脑屏障模型的起点。分析制造过程的遗传效应，以了解以这种方式封装细胞的含义。这些纤维是使用不同的制造参数来制造的，以了解对壁厚和外径的影响。然后，将多巴胺能大鼠细胞包裹成中空纤维，在为期三天的观察期内，中空纤维保持至少60%的活细胞。最后，研究了酪氨酸羟化酶(TH)和微管蛋白β3-III(Tubb-3)的遗传变化，以阐明其对细胞健康和行为的影响；尽管包埋细胞中TH的水平与对照细胞相似，显示TH合成的水平相似，但Tubb-3的表达下调，表明细胞的神经发生量更少。
At present, the blood–brain barrier (BBB) poses a challenge for treating a wide range of central nervous system disorders; reliable BBB models are still needed to understand and manipulate the transfer of molecules into the brain, thereby improving the efficiency of treatments. In this study, hollow, cell-laden microfibers are fabricated and investigated as a starting point for generating BBB models. The genetic effects of the manufacturing process are analyzed to understand the implications of encapsulating cells in this manner. These fibers are created using different manufacturing parameters to understand the effects on wall thickness and overall diameter. Then, dopaminergic rat cells are encapsulated into hollow fibers, which maintained at least 60% live cells throughout the three-day observation period. Lastly, genetic changes tyrosine hydroxylase (TH) and tubulin beta 3 class III (TUBB-3) are investigated to elucidate the effects on cell health and behavior; while the TH levels in encapsulated cells were similar to control cells, showing similar levels of TH synthesis, TUBB-3 was downregulated, indicating lower amounts of cellular neurogenesis.