PRODUCTION OF A MONOCLONAL-ANTIBODY TO AND MOLECULAR CHARACTERIZATION OF B-CELL STIMULATORY FACTOR-I
PRODUCTION OF A MONOCLONAL-ANTIBODY TO AND MOLECULAR CHARACTERIZATION OF B-CELL STIMULATORY FACTOR-I
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DOI：
10.1038/315333a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
PAUL, WE
中科院分区：
文献类型：
--
作者：
OHARA, J;PAUL, WE
B细胞刺激因子-1（BSF-1）1，以前称为B细胞生长因子，是一种T细胞衍生因子，由低浓度抗IgM抗体刺激的B细胞进入细胞周期的S期所需2 -5。BSF-1直接作用于静息B细胞，使其在随后暴露于感受态刺激时更迅速地合成DNA 6，7，并显著增强其主要组织相容性复合物II类分子的表达8，9。先前的研究表明，鼠BSF-1可与白细胞介素-2（IL-2）物理分离，该分子的表观相对分子质量（Mr）为15，000，pI值为6.4-6.7和7.4（参考文献10）。在这里，我们报告了BSF-1的单克隆抗体的生产，其在表征BSF-1中的用途，以及证明该分子不同于IL-1，IL-2和IL-3的功能研究。
B-cell stimulatory factor-1 (BSF-1)1, formerly designated B-cell growth factor, is a T-cell-derived factor required for entry into the S phase of the cell cycle by B cells stimulated with low concentrations of anti-IgM antibodies2–5. BSF-1 acts directly on resting B cells to prepare them to synthesize DNA more promptly on subsequent exposure to competent stimuli6,7and to strikingly enhance their expression of class II molecules of the major his-tocompatibility complex8,9. Previous studies have shown that murine BSF-1 can be separated physically from interleukin-2 (IL-2) and that the molecule has an apparent relative molecular mass (Mr) of ∼ 15,000 andpI values of 6.4–6.7 and 7.4 (ref. 10). Here, we report the production of a monoclonal antibody to BSF-1, its use in characterizing BSF-1, and functional studies demonstrating that this molecule is distinct from IL-1, IL-2 and IL-3.