The Arabidopsis SUMO E3 Ligase AtMMS21 Dissociates the E2Fa/DPa Complex in Cell Cycle Regulation
The Arabidopsis SUMO E3 Ligase AtMMS21 Dissociates the E2Fa/DPa Complex in Cell Cycle Regulation
复制标题
DOI：
10.1105/tpc.16.00439
复制
发表时间：
2016-09-01
期刊：
影响因子：
11.6
通讯作者：
Yang, Chengwei
中科院分区：
文献类型：
--
作者：
Liu, Yiyang;Lai, Jianbin;Yang, Chengwei
发育需要通过精确、保守的机制正确执行和调节细胞周期。重要的是，E2 F/DP复合物控制细胞周期转换期间必需基因的表达。在此，我们发现了拟南芥SUMO E3连接酶甲基甲磺酸敏感性基因21（AtMMS 21）通过E2 Fa/DPa途径调节细胞周期的分子功能。DPa被鉴定为AtMMS 21相互作用蛋白，AtMMS 21与E2 Fa竞争与DPa的相互作用。此外，DPa是AtMMS 21介导的SUMO化的底物，并且这种SUMO化增强了E2 Fa/DPa复合物的解离。AtMMS 21还影响E2 Fa/DPa的亚细胞定位。E2 Fa/DPa靶基因在mms 21 -1的根中上调，并且mms 21 -1突变体显示增加的内复制。DPa的过表达影响了mms 21 -1的根发育，AtMMS 21的过表达完全恢复了35 S：E2 Fa-DPa植株的异常表型。我们的研究结果表明AtMMS 21通过竞争和SUMO化作用解离E2 Fa/DPa复合物参与植物细胞周期的调控。
Development requires the proper execution and regulation of the cell cycle via precise, conserved mechanisms. Critically, the E2F/DP complex controls the expression of essential genes during cell cycle transitions. Here, we discovered the molecular function of the Arabidopsis thaliana SUMO E3 ligase METHYL METHANESULFONATE SENSITIVITY GENE21 (AtMMS21) in regulating the cell cycle via the E2Fa/DPa pathway. DPa was identified as an AtMMS21-interacting protein and AtMMS21 competes with E2Fa for interaction with DPa. Moreover, DPa is a substrate for SUMOylation mediated by AtMMS21, and this SUMOylation enhances the dissociation of the E2Fa/DPa complex. AtMMS21 also affects the subcellular localization of E2Fa/DPa. The E2Fa/DPa target genes are upregulated in the root of mms21-1 and mms21-1 mutants showed increased endoreplication. Overexpression of DPa affected the root development of mms21-1, and overexpression of AtMMS21 completely recovered the abnormal phenotypes of 35S: E2Fa-DPa plants. Our results suggest that AtMMS21 dissociates the E2Fa/DPa complex via competition and SUMOylation in the regulation of plant cell cycle.