Differential expression of cell adhesion molecules (CAM), neural CAM and epithelial cadherin in ependymomas and choroid plexus tumors
Differential expression of cell adhesion molecules (CAM), neural CAM and epithelial cadherin in ependymomas and choroid plexus tumors
复制标题
室管膜瘤和脉络丛肿瘤中细胞粘附分子（CAM）、神经CAM和上皮钙粘蛋白的差异表达
DOI：
10.1007/bf00309340
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发表时间：
2004
影响因子：
12.7
通讯作者：
J. Pellissier
中科院分区：
文献类型：
--
作者：
D. Figarella;H. Lepidi;C. Poncet;D. Gambarelli;N. Bianco;G. Rougon;J. Pellissier
应用多种单克隆和多克隆抗体检测了18例室管膜瘤和7例脉络丛肿瘤的冰冻标本中细胞粘附分子（如神经细胞粘附分子（NCAM）、其多聚唾液酸化亚型（PSA NCAM）和上皮（E-）钙粘蛋白）以及中间丝蛋白（如胶质细胞酸性蛋白（GFAP）和细胞角蛋白）的表达。正常脉络丛和室管膜作为对照。大多数成人脉络丛和良性脉络丛乳头状瘤细胞的基底外侧部可见抗E-钙粘蛋白免疫反应。然而，少数非典型乳头状瘤和癌细胞显示抗E-钙粘蛋白免疫反应性遍及其细胞表面膜。成人脉络丛和良性乳头状瘤细胞不表达NCAM。在发育中的脉络丛、不典型乳头状瘤和癌中只有少数细胞表达NCAM和PSA NCAM。脉络丛及其肿瘤中均可见细胞角蛋白表达，GFAP表达则因病例而异。相反，室管膜细胞及其肿瘤从不表达E-钙粘蛋白，但强烈表达NCAM。PSA NCAM见于表现间变性特征的室管膜瘤。所有室管膜瘤均强表达GFAP，少数室管膜瘤表达细胞角蛋白。这些数据表明，除了GFAP和细胞角蛋白，NCAM和E-cadherin是潜在的诊断价值，以区分脉络丛肿瘤和室管膜瘤。E-cadherin和NCAM可能分别在正常脉络丛和室管膜的功能组织中发挥作用。特别是，脉络丛肿瘤中抗E-钙粘蛋白的不完全或不规则表达以及室管膜瘤和脉络丛肿瘤中PSA NCAM免疫反应性与间变性组织学特征的出现相关。
A series of frozen specimens of 18 ependymomas and 7 choroid plexus tumors were examined for their expression of cell adhesion molecules, such as neural cell adhesion molecule (NCAM), its polysialylated isoforms (PSA NCAM), and epithelial (E-) cadherin, and of intermediate filament proteins, such as glial fibrillary acidic protein (GFAP) and cytokeratin, using various monoclonal and polyclonal antibodies. Normal choroid plexus and ependyma were taken as controls. Anti-E-cadherin immunoreactivity was observed on the basolateral part of most adult choroid plexus and benign choroid plexus papilloma cells. However, a small number of atypical papillomas and carcinoma cells showed anti- E-cadherin immunoreactivity throughout their cell surface membrane. NCAM were not expressed by adult choroid plexus and benign papilloma cells. Only a few cells expressed NCAM and PSA NCAM in developing choroid plexus, atypical papillomas and carcinomas. Cytokeratin expression was always observed in choroid plexus and their tumors; GFAP expression was variable from case to case. In contrast, ependymal cells and their tumors never expressed E-cadherin but strongly expressed NCAM. PSA NCAM was found in ependymomas exhibiting anaplastic features. All ependymomas strongly expressed GFAP and a few demonstrated slight expression of cytokeratin. These data suggest that, besides GFAP and cytokeratin, NCAM and E-cadherin are of potential diagnostic value in distinguishing choroid plexus tumors from ependymomas. E-cadherin and NCAM may play a role in the functional organization of normal choroid plexus and ependyma, respectively. In particular, incomplete or irregular anti-E-cadherin expression in choroid plexus tumors and PSA NCAM immunoreativity in ependymomas and choroid plexus tumors correlates with the emergence of anaplastic histological features.
影响因子：
2.7
作者：
Diane M. Snow;Dennis A. Steindler;Jerry Silver
通讯作者：
Diane M. Snow;Dennis A. Steindler;Jerry Silver