ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy.
ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy.
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DOI：
10.1038/srep24084
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发表时间：
2016-04-12
影响因子：
4.6
通讯作者：
Klaholz BP
中科院分区：
文献类型：
--
作者：
Andronov L;Orlov I;Lutz Y;Vonesch JL;Klaholz BP
超分辨率显微镜（PALM、STORM等）在密集的细胞环境中提供过多的荧光信号，这可能难以解释。在这里，我们描述的图像重建，可视化和定量标记的蛋白质簇的方法，基于Voronoi镶嵌的个别荧光事件。这种聚类方法的超分辨率显微镜数据的分割，包括共定位的一般适用性，说明了一系列重要的生物对象，如染色质复合物，RNA聚合酶，核孔复合物和微管。
Super-resolution microscopy (PALM, STORM etc.) provides a plethora of fluorescent signals in dense cellular environments which can be difficult to interpret. Here we describe ClusterViSu, a method for image reconstruction, visualization and quantification of labelled protein clusters, based on Voronoi tessellation of the individual fluorescence events. The general applicability of this clustering approach for the segmentation of super-resolution microscopy data, including for co-localization, is illustrated on a series of important biological objects such as chromatin complexes, RNA polymerase, nuclear pore complexes and microtubules.