Hepatic Sirt3 expression declines postpartum in dairy goats
Hepatic Sirt3 expression declines postpartum in dairy goats
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奶山羊产后肝脏 Sirt3 表达下降。
DOI：
10.1017/s0022029918000171
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发表时间：
2018-05-01
影响因子：
2.1
通讯作者：
He, Jianhua
中科院分区：
文献类型：
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作者：
Liu, Lei;Yao, Li;He, Jianhua
本研究通讯中报道的实验旨在绘制Sirt 3的表达模式，Sirt 3是能量代谢和抗氧化防御的主要调节因子，在围产期奶山羊肝脏中。选择10只妊娠晚期健康奶山羊，每隔1周进行一次肝脏穿刺活检。通过蛋白质印迹分析肝Sirt 3的蛋白质水平。测定血清锰超氧化物歧化酶（Mn-SOD）活性和非酯化脂肪酸（NEFA）水平，并分析其与Sirt 3 mRNA水平的相关性。Sirt 3蛋白在分娩后1周和2周较分娩前3周显著降低（P
The experiments reported in this research communication aimed to plot the expression pattern of Sirt3, a master regulator of energy metabolism and antioxidation defence, in the liver of dairy goats during perinatal period. Ten healthy dairy goats in late pregnancy were chosen, and needle biopsy was applied to collect liver samples at 1-week intervals. Protein levels of hepatic Sirt3 were analysed by western-blotting. Serum enzyme activities of manganese superoxide dismutase (Mn-SOD) and non-esterified fatty acids (NEFA) levels were measured, and their correlation with Sirt3 mRNA levels was also estimated. Compared with >3-week before parturition (BP), Sirt3 proteins were significantly reduced at 1-week after parturition (AP) and 2-week AP (P