Analysis of 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation
Analysis of 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation
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DOI：
10.2436/20.1501.01.59
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发表时间：
2008-09-01
影响因子：
3.1
通讯作者：
Martinez-Murcia, Antonio J.
中科院分区：
文献类型：
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作者：
Alperi, Anabel;Figueras, Maria J.;Martinez-Murcia, Antonio J.
使用已发表的16 S rDNA RFLP鉴定方法对999株气单胞菌菌株进行表征，结果显示8.1%的菌株产生了意想不到的（以下称为“非典型”）限制性图谱，使其鉴定不确定。非典型模式是由于16 S rRNA基因的rrn操纵子之间存在核苷酸多态性（所谓的微异质性）。在某些位置的双测序信号揭示了核苷酸组成是负责微异质性。虽然微异质性的数量相对较低（0.06-0.66%），从16 S rRNA基因推断的树导致错误鉴定或对大多数这些菌株的不确定结果。然而，使用rpoD基因序列，具有非典型模式的菌株被正确鉴定为属于豚鼠气单胞菌，A. veronii和A.媒体所有这些物种，尤其是前两个物种，都与人类疾病有关。临床菌株16 SrRNA基因微变异率显著高于环境菌株（P < 0.01）。本工作还分析了这些微异质性对所调查菌株的分类地位的影响。结果表明，需要记录16 S rRNA基因的微异质性。[Int微生物学2008; 11（3）：185-194]
Characterization of 999 Aeromonas strains using a published 16S rDNA RFLP identification method showed that 8.1% of the strains produced unexpected (hereafter called "atypical") restriction patterns, making their identification uncertain. Atypical patterns were due to the presence of nucleotide polymorphisms among the rrn operons of the 16S rRNA gene (so-called microheterogeneities). Double sequencing signals at certain positions revealed the nucleotide composition was responsible for the microheterogeneities. Although the number of microheterogeneities was relatively low (0.06-0.66%), trees inferred from the 16S rRNA gene led either to a misidentification or to an inconclusive result for the majority of these strains. Strains with atypical patterns were, however, correctly identified using the rpoD gene sequences, as belonging to Aeromonas caviae, A. veronii, and A. media. All of them, but particularly the two former species, are associated with human disease. Microheterogeneities in 16S rRNA gene sequence were significantly (P < 0.01) more prevalent in clinical than in environmental strains. This work also analyzed the effects of these microheterogeneities on the taxonomic position of the investigated strains. The results suggest the need for recording microheterogeneities in the 16S rRNA gene. [Int Microbiol 2008; 11(3):185-194]