FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development
FMR1/FXR1 and the miRNA pathway are required for eye and neural crest development
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DOI：
10.1016/j.ydbio.2010.02.031
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发表时间：
2010-05-01
影响因子：
2.7
通讯作者：
Kuehl, Michael
中科院分区：
文献类型：
--
作者：
Gessert, Susanne;Bugner, Verena;Kuehl, Michael
FMR1 和 FXR1 是 RNA 结合蛋白，与 miRNA 诱导的沉默复合物 RISC 相互作用。在这里，我们首次描述了这些蛋白质在非洲爪蟾眼睛和神经嵴（NC）发育过程中的功能。 FMR1 或 FXR1 缺失会导致眼睛发育异常以及颅 NC 细胞衍生的颅软骨缺陷。我们进一步研究了这些表型的可能机制，表明前神经组织中 Dicer（一种生成所有成熟 miRNA 的重要酶）的消耗也会导致眼睛和颅骨软骨缺陷。此外，我们检查了 12 种 miRNA 在前神经发育过程中的功能。我们展示了眼睛和颅软骨发育过程中六种选定的 miRNA 的特定需求。 Mir-130a、-219 和 -23b 仅参与眼睛形成，而 miR-200b、miR-96 和 miR-196a 的缺失会导致眼睛和颅软骨发育过程中的严重缺陷。我们的结果表明，FMR1 和 FXR1 可能通过与 miRNA 途径相互作用，对非洲虎眼睛和 NC 发育发挥重要作用。 (C) 2010 Elsevier Inc. 保留所有权利。
FMR1 and FXR1 are RNA binding proteins interacting with the miRNA-induced silencing complex, RISC. Here we describe for the first time the function of these proteins during eye and neural crest (NC) development in Xenopus laevis. A loss of FMR1 or FXR1 results in abnormal eye development as well as defects in cranial cartilage derived from cranial NC cells. We further investigated the possible mechanism of these phenotypes by showing that a depletion of Dicer, an important enzyme for generating all mature miRNAs, in the anterior neural tissue also leads to eye and cranial cartilage defects. Furthermore, we examined the function of 12 miRNAs during anterior neural development. We show a specific requirement of six selected miRNAs during eye and cranial cartilage development. Mir-130a, -219, and -23b are involved in eye formation only whereas loss of miR-200b, miR-96 and miR-196a results in strong defects during eye as well as cranial cartilage development. Our results suggest an essential role for FMR1 and FXR1 for eye and NC development in X. laevis likely through an interaction with the miRNA pathway. (C) 2010 Elsevier Inc. All rights reserved.