Tea pigments inhibit the production of type 1 (TH1) and type 2 (TH2) helper T cell cytokines in CD4+ T cells
Tea pigments inhibit the production of type 1 (TH1) and type 2 (TH2) helper T cell cytokines in CD4+ T cells
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DOI：
10.1002/ptr.834
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发表时间：
2002-02-01
影响因子：
7.2
通讯作者：
Santoro, TJ
中科院分区：
文献类型：
--
作者：
Tomita, M;Irwin, KI;Santoro, TJ
茶色素是茶叶中多酚类物质的氧化产物。茶黄素是茶色素的主要成分，具有与其母体化合物相似的抗氧化、抗氧化和抗氧化特性。多酚和茶黄素的生物学特性与它们抑制核因子-κ B（NF-κ B）活化的能力有关，核因子-κ B是一种转录因子，其关键地参与许多促炎细胞因子的分子调节。目前的研究探讨了茶抗氧化剂介导的免疫抑制作用对NF-κ B的需求。具体来说，我们测试了这样的假设，即由1型（T-H1）CD 4（1）T细胞产生的细胞因子（需要NF-κ B进行基因表达），如白细胞介素-2（IL-2）和干扰素γ（IFN γ），被茶色素选择性抑制。我们发现，茶色素有效地抑制IL-2分泌，IL-2基因表达和NF-κ B在小鼠脾脏富集的CD 4（1）T细胞的激活，正如预期的那样。与我们的假设一致，茶色素也抑制IFN γ mRNA的诱导。然而，T-H2细胞因子IL-4和IL-5的表达，其启动子内缺乏功能性NF-κ B位点，也意外地被茶色素抑制。结果表明，NF-κ B可能只是茶色素抑制的多种转录因子之一。版权所有（C）2002约翰威利父子有限公司。
Tea pigments are oxidized products of polyphenols derived front tea leaves (Camellia sinensis). Theaflavins are constituents of tea pigments with antioxidant, antineoplastic and antiinflammatory properties similar to their parent compounds. The biological properties of polyphenols and theaflavins have been linked to their capacity to inhibit the activation of nuclear factor-kappaB (NF-kappaB), a transcription factor, which is critically involved in the molecular regulation of a number of proinflammatory cytokines. The current study examines the requirement for NF-kappaB in the immunosuppressive effects mediated by tea antioxidants. Specifically, we tested the hypothesis that cytokines produced by type 1 (T-H1) CD4(1) T cells which require NF-kappaB for gene expression, such as interleukin-2 (IL-2) and interferon gamma (IFN gamma), are selectively inhibited by tea pigments. We found that tea pigments potently suppress IL-2 secretion, IL-2 gene expression and the activation of NF-kappaB in murine spleens enriched for CD4(1) T cells, as expected. Consistent with our hypothesis, tea pigments also inhibited the induction of IFNgamma mRNA. However, the expression of the T-H2 cytokines IL-4 and IL-5, which lack functional NF-kappaB sites within their promoters was unexpectedly suppressed by tea pigments, as well. The results indicate that NF-kappaB may be only one of multiple transcription factors inhibited by tea pigments. Copyright (C) 2002 John Wiley and Sons, Ltd.