A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species

A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species
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DOI:
10.1021/jf072975
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发表时间:
2008-04-09
影响因子:
6.1
通讯作者:
Ferreres, Federico
Ferreres, Federico
中科院分区:
农林科学1区
文献类型:
--
作者:
Martinez-Sanchez, Ascension;Gil-Izquierdo, Angel;Ferreres, Federico

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摘要对四种用于制作沙拉的芸苔科蔬菜——水菜(Nasturtium officinale R. Br.)、水菜(mizuna [Brassica rapa L. subsp.])的抗氧化成分、黄酮类化合物和维生素C及其抗氧化活性进行了比较研究。尼波西尼(L.H. Bailey) hanelland),野火箭[Diplotaxis tenuifolia (L.)]直流。]和沙拉火箭[花椰菜(L.)]骑兵)。采用HPLC-DAD-MS/MS-ESI对豆瓣菜和水菜中单个酚类化合物进行了表征,完成了先前报道的野生火箭和沙拉火箭中多酚类化合物的研究。对豆瓣菜叶片中黄酮类化合物的定性研究表明,在7号位置有鼠李糖的糖基化特征。在水豆叶片中鉴定出了鼠李素3,7-二- o -葡萄糖苷,可以认为是一些rapa亚种的化学分类标记。十字花科植物中黄酮类化合物含量的定量研究存在差异,以豆瓣菜叶片含量最高。不同方法(ABTS、DPPH和FRAP)测定的抗氧化活性与多酚和维生素c含量呈高度相关。由此可见,所研究的十字花科菜叶(豆瓣菜、水菜、野生菜叶和沙拉菜叶)抗氧化化合物的组成和含量存在较大差异。这些幼叶种是抗氧化剂的良好膳食来源,具有重要的生物活性化合物变异性。
A comparative study of antioxidant compounds, flavonoids and vitamin C, and also antioxidant activity was carried out in four species of Brassicaceae vegetables used for salads: watercress (Nasturtium officinale R. Br.), mizuna [Brassica rapa L. subsp. nipposinica (L.H. Bailey) Haneltand], wild rocket [Diplotaxis tenuifolia (L.) DC.], and salad rocket [Eruca vesicaria (L.) Cav]. The characterization of individual phenolic compounds by HPLC-DAD-MS/MS-ESI in watercress and mizuna completes the polyphenol study previously reported for wild rocket and salad rocket. The qualitative study of flavonoids in watercress leaves showed a characteristic glycosylation pattern with rhamnose at the 7 position. lsorhamnetin 3,7-di-O-glucoside was identified in mizuna leaves and may be considered a chemotaxonomical marker in some B. rapa subspecies. Brassicaceae species showed differences in the quantitative study of flavonoids, and the highest content was detected in watercress leaves. Watercress and wild rocket leaves had the highest content of vitamin C. The antioxidant activity evaluated by different methods (ABTS, DPPH, and FRAP assays) showed a high correlation level with the content of polyphenols and vitamin C. In conclusion, the Brassicaceae leaves studied, watercress, mizuna, wild rocket, and salad rocket, presented a large variability in the composition and content of antioxidant compounds. These baby leaf species are good dietary sources of antioxidants with an important variability of bioactive compounds.