Genetic polymorphisms in porcine leptin gene and their association with reproduction and production traits
Genetic polymorphisms in porcine leptin gene and their association with reproduction and production traits
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DOI：
10.1071/ar03258
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发表时间：
2004-07
影响因子：
1.9
通讯作者：
Chi-Chen Chen-Chi-Chen-Chen-2109612899;Tsern-Shi Chang;H. Su
中科院分区：
文献类型：
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作者：
Chi-Chen Chen-Chi-Chen-Chen-2109612899;Tsern-Shi Chang;H. Su
瘦素（LEP）是瘦素基因的产物，是由脂肪组织合成的16-kDa蛋白，并且参与调节摄食、能量平衡、生育和免疫功能。采用双向聚合酶链反应扩增特异等位基因（bi-PASA）和PCR-RFLP技术，分别检测了长白猪、约克郡猪和杜洛克猪LEP基因第1内含子和第3外显子的遗传多态性。采用卡方统计方法计算3个品种2个候选基因的基因型频率的同质性。第3外显子多态性对长白猪第1胎和1-4胎产羔数等繁殖性状的影响显著（P < 0.05），对约克郡猪1-4胎产羔数的影响显著（P < 0.05）。内含子1多态性仅对杜洛克猪第一胎产羔数有显著影响（P < 0.05）。长白猪和约克郡猪外显子3多态性对日增重和饲料效率等生产性状的影响显著（P < 0.05）。内含子1多态性仅对杜洛克猪背膘厚有显著影响（P < 0.05）。我们的数据还表明，繁殖性状越高，基因型频率越低。生产性状越好，基因型频率越高。这些数据表明，猪生产中有利性状的长期选择可能部分归因于LEP多态性对繁殖和生产性状的影响。
Leptin (LEP), the product of the leptin gene, is a 16-kDa protein synthesised by adipose tissue and is involved in regulation of feed intake, energy balance, fertility, and immune functions. Using bi-directional polymerase chain reaction amplification of specific alleles (bi-PASA) and PCR-restriction fragment length polymorphisms (PCR-RFLP), we have successfully detected genetic polymorphisms of LEP intron 1 and exon 3, respectively, in Landrace, Yorkshire, and Duroc. Chi-square statistics were used to calculate homogeneity of genotypic frequencies of 2 candidate genes for 3 breeds of animals. Effects of exon 3 polymorphisms on reproduction traits such as litter sizes of sows in the first parity and 1-4 parities were evident (P < 0.05) in Landrace, and 1-4 parities in Yorkshire. Polymorphisms of intron 1 affected (P < 0.05) only litter sizes of the first parity in Duroc. Effects of exon 3 polymorphisms on production traits such as average daily gain and feed efficiency were evident (P < 0.05) in Landrace and Yorkshire, respectively. Polymorphisms of intron 1 affected (P < 0.05) only backfat thickness in Duroc. Our data also show that the higher the reproduction traits, the lower the genotypic frequencies. Further, the better the production traits, the higher the genotypic frequencies were. These data suggest that the long- term selection of favourable traits in pig production may be partially attributed to the effects of LEP polymorphisms on reproduction and production traits.