EFFECT OF NEUTRAL POLYMERS ON ELECTROKINETIC POTENTIAL OF CELLS AND OTHER CHARGED-PARTICLES .3. EXPERIMENTAL STUDIES ON DEXTRAN-ERYTHROCYTE SYSTEM
EFFECT OF NEUTRAL POLYMERS ON ELECTROKINETIC POTENTIAL OF CELLS AND OTHER CHARGED-PARTICLES .3. EXPERIMENTAL STUDIES ON DEXTRAN-ERYTHROCYTE SYSTEM
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DOI：
10.1016/0021-9797(73)90415-3
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发表时间：
1973-01-01
影响因子：
9.9
通讯作者：
BROOKS, DE
中科院分区：
文献类型：
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作者：
BROOKS, DE
的实验检查是由葡聚糖/红细胞悬浮液的目的是测试以前提出的这些系统的电动行为的理论。右旋糖酐被证明吸附到正常人红细胞从简单的盐溶液中，吸附量随右旋糖酐浓度在很宽的范围内线性变化。发现葡聚糖中细胞的相对zeta电位Z取决于存在的单价抗衡离子的种类，但不取决于共离子。Z对离子强度的依赖关系的测量和示出同意与理论表达式推导出先前为一个适当的选择参数。这样获得的参数以从这些和其他系统中的聚合物吸附特性中预期的方式变化。特别是，通过解释电泳结果在我们的理论，和葡聚糖吸附测量的吸附参数之间的直接比较提供了大量的支持，在检查的条件范围内的模型。
An experimental examination is made of dextran/erythrocyte suspensions with the aim of testing the theory previously proposed for the electrokinetic behavior of these systems. Dextran is shown to adsorb to normal human erythrocytes from simple salt solutions; the amount adsorbed varies linearly with dextran concentration over a wide range. The relative zeta potential, Z, of cells in dextran is found to depend on the species of monovalent counterion present but not the co-ion. The dependence of Z on ionic strength is measured and shown to agree with the theoretical expression derived previously for an appropriate choice of parameters. The parameters so obtained vary in a manner which is anticipated from the characteristics of polymer adsorption in these and other systems. In particular, a direct comparison between the adsorption parameter, obtained by interpreting the electrophoretic results in terms of our theory, and the dextran adsorption measurements provides substantial support for the model over the range of conditions examined.