Diversity of HIV-1 RNA and DNA in breast milk from HIV-1-infected mothers
Diversity of HIV-1 RNA and DNA in breast milk from HIV-1-infected mothers
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DOI：
10.1016/j.virol.2007.02.003
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发表时间：
2007-07-05
期刊：
影响因子：
3.7
通讯作者：
Van de Perre, Philippe
中科院分区：
文献类型：
--
作者：
Becquart, Pierre;Courgnaud, Valerie;Van de Perre, Philippe
我们比较了人类免疫缺陷病毒1型（HIV-1）的RNA和DNA群体的不同部分的母乳（乳清，脂质层，细胞团）和左右乳房之间的4个HIV-1感染的母亲通过分析高变的env C2-V5区。系统发育分析的病毒准种显示，RNA群体和DNA群体是明显不同的，病毒RNA序列相似的脂质层和乳清中的牛奶中的3个4母亲。左右乳房乳汁中病毒DNA的比较显示，三位母亲的变异体分布不同。相比之下，从两个乳房的乳汁中检测到的RNA变体在4个母亲中的3个中混合。这项研究表明，每个乳腺受到微环境压力，可能不同于对侧乳房。(C)2007年由Elsevier Inc.出版
We compared human immunodeficiency virus type 1 (HIV-1) RNA and DNA populations in the different fractions of breast milk (lactoserum, lipid layer, cell pellet) and between right and left breasts in four HIV-1-infected mothers by analyzing the hypervariable env C2-V5 region. Phylogenetic analyses of the viral quasispecies revealed that RNA populations and DNA populations were clearly distinct and that viral RNA sequences were similar in lipid layer and lactoserum in the milk of 3 out of 4 mothers. Comparison of viral DNA between milk from right and left breast showed a differential distribution of variants in three mothers. In contrast, RNA variants detected from milk of the two breasts were mixed in 3 out of 4 mothers. This study suggests that each mammary gland is subjected to microenvironmental pressure that may differ from the contralateral breast. (C) 2007 Published by Elsevier Inc.