Proteomic and Microscopic Strategies towards the Analysis of the Cytoskeletal Networks in Major Neuropsychiatric Disorders.
Proteomic and Microscopic Strategies towards the Analysis of the Cytoskeletal Networks in Major Neuropsychiatric Disorders.
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DOI：
10.3390/ijms17040581
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发表时间：
2016-04-20
影响因子：
5.6
通讯作者：
Moens PD
中科院分区：
文献类型：
--
作者：
Coumans JV;Palanisamy SK;McFarlane J;Moens PD
精神健康障碍已成为世界范围内的健康优先事项。据估计，在未来20年内，它们将造成16万亿美元(美元)的损失。到目前为止，精神障碍的潜在病理生理学仍然难以捉摸。细胞骨架蛋白表达的改变可能影响细胞骨架的组装、组织和维持完整性，已在严重的抑郁症、精神分裂症和一定程度上的双相情感障碍中得到报道。使用定量蛋白质组学、动态显微镜和超分辨率显微镜来研究疾病特异性蛋白质特征具有很大的希望，可以提高我们对这些疾病的理解。在这篇综述中，我们介绍了目前可用的定量蛋白质组学方法在神经病学、基于凝胶的、稳定的同位素标记和无标记方法中的应用，并评价了它们的优点和局限性。我们还报道了以细胞骨架相关蛋白为靶点的浓缩/亚分离方法，并讨论了进一步鉴定神经细胞骨架蛋白质组的替代方法的必要性。最后，我们介绍了活细胞成像方法和新兴的动态显微镜技术，它们将提供必要的工具来研究整个细胞中的蛋白质相互作用及其动力学。虽然这些领域的研究仍处于初级阶段，但它们为理解神经网络稳定性及其跨神经精神障碍的修饰提供了巨大的潜力。
Mental health disorders have become worldwide health priorities. It is estimated that in the next 20 years they will account for a 16 trillion United State dollars (US$) loss. Up to now, the underlying pathophysiology of psychiatric disorders remains elusive. Altered cytoskeleton proteins expression that may influence the assembly, organization and maintenance of cytoskeletal integrity has been reported in major depressive disorders, schizophrenia and to some extent bipolar disorders. The use of quantitative proteomics, dynamic microscopy and super-resolution microscopy to investigate disease-specific protein signatures holds great promise to improve our understanding of these disorders. In this review, we present the currently available quantitative proteomic approaches use in neurology, gel-based, stable isotope-labelling and label-free methodologies and evaluate their strengths and limitations. We also reported on enrichment/subfractionation methods that target the cytoskeleton associated proteins and discuss the need of alternative methods for further characterization of the neurocytoskeletal proteome. Finally, we present live cell imaging approaches and emerging dynamic microscopy technology that will provide the tools necessary to investigate protein interactions and their dynamics in the whole cells. While these areas of research are still in their infancy, they offer huge potential towards the understanding of the neuronal network stability and its modification across neuropsychiatric disorders.