Cold plasma enzyme inactivation on dielectric properties and freshness quality in bananas

Cold plasma enzyme inactivation on dielectric properties and freshness quality in bananas
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DOI:
10.1016/j.ifset.2021.102649
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发表时间:
2021-03-06
影响因子:
6.6
通讯作者:
Jiang, Hao
Jiang, Hao
中科院分区:
农林科学1区
文献类型:
--
作者:
Gu, Yuxiang;Shi, Wenqing;Jiang, Hao

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冷等离子体(CP)技术是一种新型的、非热的灭活酶、改善食品原料品质的方法。在本研究中,香蕉样品经过CP处理不同时间(间隔1、1.5和2min),测量其介电特性、酶活性、ROS水平、酶促褐变产物、颜色、风味物质和表面微结构。CP处理后的介电常数(epsilon‘)和损耗因数(epsilon’‘)分别随频率的升高而减小,随温度的升高而增大。CP处理后也有较高的epsilon‘和epsilon’‘值。多酚氧化酶(PPO)和过氧化物酶(POD)活性随CP处理时间的延长而降低。CP处理后细胞内ROS水平升高,酶促褐变产生的苯二酚随着处理时间的延长而减少。由于酶失活,CP处理对香蕉的表面颜色有积极的影响,但不同处理时间的a*和b*值存在显著差异。然而,随着CP加工时间的延长,表面形态发生变化,导致表面组织变得更加粗糙。CP处理对风味物质影响不大,是一种潜在的介电加热/干燥前处理技术。
Cold plasma (CP) technology is a novel, non-thermal method to inactive enzymes and improve the quality of food materials. In this study, banana samples were processed via CP treatment for different lengths of time (1, 1.5, and 2 min intervals) and their properties, including dielectric properties, enzyme activities, levels of ROS (reactive oxygen species), enzymatic browning products, color, flavor compounds, and surface microstructure, were measured. The dielectric constant (epsilon') and loss factor (epsilon '') after CP treatment decreased with increasing frequency and increased with increasing temperature, respectively. Higher values of epsilon' and epsilon '' could also be found after CP treatment. The enzyme activity of polyphenoloxidase (PPO) and peroxydase (POD) decreased with increasing CP treatment time. ROS levels in cells increased after CP treatment, and quinones generated from enzymatic browning decreased at extended treatment times. CP treatment had a positive effect on the surface color of bananas due to enzyme inactivation, while significant differences in a* and b* values at different treatment times were noted. However, surface morphology changed with increasing CP processing time, leading to a rougher surface microstructure. CP treatment has little impact on flavor compounds and has demonstrated a potential pretreatment technology for dielectric heating/drying.