Expression of the simian varicella virus glycoprotein L and H
Expression of the simian varicella virus glycoprotein L and H
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DOI：
10.1007/s705-002-8323-6
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Gray, WL
中科院分区：
文献类型：
--
作者：
Ashburn, CV;Gray, WL
猴水痘是研究水痘-带状疱疹病毒致病机制和评价抗病毒治疗的模型。在这项研究中，猴水痘病毒（SVV）的糖蛋白L（gL）的特点是沿着与糖蛋白H（gH）。SVV gL基因编码预测的175个氨基酸的多肽，其分别与VZV gL和HSV-1 gL共享43.5%和27.9%的氨基酸同一性。SVV gL多肽序列缺乏共有糖基化位点和典型的信号序列，但确实具有在伴侣蛋白中常见的内质网靶向序列。转录分析表明，SVV gL和尿嘧啶DNA糖基化酶（UDG）基因共享一个共同的5' RNA起始位点，并在一个2.0 kb的转录本上共表达。使用针对gL和gH肽产生的特异性抗血清，通过免疫荧光和免疫沉淀分析证实了SVV感染的Vero细胞中gL和gH的表达。与其他疱疹病毒gH和gL同源物类似，SVV gL和gH在感染细胞内形成复合物。在猴水痘急性期组织中检测到gL和gH的转录本和抗原。猴水痘模型提供了一个机会，研究gL和gH在病毒发病机制中的作用。
Simian varicella is used as a model to investigate varicella-zoster virus pathogenesis and to evaluate antiviral therapies. In this study, the simian varicella virus (SVV) glycoprotein L (gL) was characterized along with its association with glycoprotein H (gH). The SVV gL gene encodes a predicted 175 amino acid polypeptide that shares 43.5% and 27.9% amino acid identity with the VZV gL and HSV-1 gL, respectively. The SVV gL polypeptide sequence lacks a consensus glycosylation site and a typical signal sequence, but does possess an endoplasmic reticulum targeting sequence found commonly in chaperone proteins. Transcriptional analysis indicated that the SVV gL and the uracil DNA glycosylase (UDG) genes share a common 5' RNA start site and are co-expressed on a 2.0 kb transcript. gL and gH expression in SVV-infected Vero cells was demonstrated by immunofluorescence and immunoprecipitation analyses using specific antisera generated against gL and gH peptides. Similar to other herpesvirus gH and gL homologs, the SVV gL and gH form a complex within infected cells. gL and gH transcripts and antigens were detected in tissues of monkeys with acute simian varicella. The simian varicella model offers an opportunity to investigate the role of the gL and gH in viral pathogenesis.