Circular and linear simian virus 40 DNAs differ in recombination.
Circular and linear simian virus 40 DNAs differ in recombination.
复制标题
环状和线性猿猴病毒 40 DNA 在重组方面有所不同。
DOI：
10.1128/mcb.5.4.869-880.1985
复制
发表时间：
1985
影响因子：
5.3
通讯作者：
Winocour,E
中科院分区：
文献类型：
--
作者：
Dorsett,D;Deichaite,I;Winocour,E
Linear forms of simian virus 40 (SV40) DNA, when added to transfection mixtures containing circular SV40 and ϕX174 RFI DNAs, enhanced the frequency of SV40/ϕX174 recombination, as measured by infectious center in situ plaque hybridization in monkey BSC-1 cells. The sequences required for the enhancement of recombination by linear DNA reside within the SV40 replication origin/regulatory region (nucleotides 5,171 to 5,243/0 to 128). Linearization of ϕX174 RFI DNA did not increase the recombination frequency. The SV40/ϕX174 recombinant structures arising from transfections supplemented with linear forms of origin-containing SV40 DNA contained ϕX174 DNA sequences interspersed within tandem head-to-tail repeats derived from the recombination-enhancing linear DNA. Evidence is presented that the tandem repeats are not formed by homologous recombination and that linear forms of SV40 DNA must compete with circular SV40 DNA for the available T antigen to enhance recombination. We propose that the enhancement of recombination by linear SV40 DNA results from the entry of that DNA into a rolling circle type of replication pathway which generates highly recombinogenic intermediates.
