Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture
Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono- and Co-Culture
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DOI：
10.1080/15287394.2015.1043607
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发表时间：
2015-08-03
影响因子：
2.6
通讯作者：
Shim, Soon-Mi
中科院分区：
文献类型：
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作者：
Kim, Ju-Hyeong;Cho, Myung-Haing;Shim, Soon-Mi
本研究的目的是研究香烟烟雾提取物（CSE）或香烟烟雾冷凝物（CSC）诱导的氧化应激在人脑细胞（T98 G）和人脑微血管内皮细胞（HBMEC）在单一和共培养系统。与CSE（0.025-20%）相比，暴露于CSC（0.05-4 mg/ml）的T98 G细胞的细胞活力显著降低。与对照组相比，CSE（2、4和10%）或CSC（0.2、0.4和0.8 mg/ml）处理的活性氧（ROS）生成量无显著差异。然而，在4 mg/ml CSC孵育后，注意到ROS产生的显著影响。在CSE和CSC的IC 50值下，HBMEC的细胞完整性在暴露120 h内分别降低至74%和64%。这项研究表明，长期暴露于吸烟可能会引起血脑屏障的损害。
The objective of the current study was to examine oxidative stress induced by cigarette smoke extract (CSE) or cigarette smoke condensate (CSC) in human brain cells (T98G) and human brain microvascular endothelial cells (HBMEC) in mono- and co-culture systems. Cell viability of T98G cells exposed to CSC (0.05-4 mg/ml) was significantly decreased compared to CSE (0.025-20%). There were no marked differences between quantities of reactive oxygen species (ROS) generation by either CSE (2, 4, and 10%) or CSC (0.2, 0.4, and 0.8 mg/ml) treatment compared to control. However, a significant effect was noted in ROS generation following CSC incubation at 4mg/ml. Cellular integrity of HBMEC decreased to 74 and 64% within 120 h of exposure at the IC50 value of CSE and CSC, respectively. This study suggests that chronic exposure to cigarette smoking might initiate damage to the blood-brain barrier.