Realtime visualization of tumor cell/endothelial cell interactions during transmigration across the endothelial barrier
Realtime visualization of tumor cell/endothelial cell interactions during transmigration across the endothelial barrier
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DOI：
10.1007/s00432-002-0377-7
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发表时间：
2002-10-01
影响因子：
3.6
通讯作者：
Dittmar, T
中科院分区：
文献类型：
--
作者：
Heyder, C;Gloria-Maercker, E;Dittmar, T
目的：在癌症中，肿瘤细胞的血液传播导致在远处位点形成继发性肿瘤，由此肿瘤细胞的外渗是血行转移期间的先决条件。在这里，我们描述了一种新的体外实时模型，它显示了外渗过程的完整序列。研究方法：我们开发了一种体外系统，使我们能够监测肿瘤细胞簇跨单层人脐带内皮细胞（HUVEC）外渗事件的顺序。荧光标记和激光扫描共聚焦显微镜用于显示肿瘤细胞和内皮之间的相互作用。结果如下：我们的模型表明，外渗的肿瘤细胞团来自浸润性人膀胱癌细胞系T24发生在一个相对较短的时间框架后，粘附到内皮细胞4小时。我们证明，血管内皮是不可逆的损害，在网站的肿瘤细胞外渗。结论：实时激光扫描共聚焦显微镜导致更好地了解外渗过程中复杂和动态的细胞与细胞和细胞与基质的相互作用。
Purpose: In cancer the blood-borne spread of tumor cells leads to the formation of secondary tumors at distant loci whereby the extravasation of tumor cells is a prerequisite step during hematogenous metastasis. Here, we describe a novel in vitro realtime model which shows the complete sequence of the extravasation process. Methods: We developed an in vitro system allowing us to monitor the sequence of extravasation events of tumor cell clusters across a monolayer of human umbilical cord endothelial cells (HUVEC). Fluorescence markers and laser scanning confocal microscopy were used to visualize the interactions between tumor cells and endothelium. Results: Our model indicates that the extravasation of tumor cell clusters derived from the invasive human bladder carcinoma cell line T24 occurs in a relatively short time-frame up to 4 h after adhesion to the endothelium. We demonstrate that the vascular endothelium is irreversibly damaged at the site of tumor cell extravasation. Conclusion: Real-time laser scanning confocal microscopy leads to a better understanding of the complex and dynamic cell-to-cell and cell-to-matrix interactions during the extravasation process.