Stage-Specific Expression of Smad2 and Smad3 During Folliculogenesis1
Stage-Specific Expression of Smad2 and Smad3 During Folliculogenesis1
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DOI：
10.1095/biolreprod66.6.1571
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发表时间：
2002-06
影响因子：
8.7
通讯作者：
Jian Xu;J. Oakley;E. Mcgee
中科院分区：
文献类型：
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作者：
Jian Xu;J. Oakley;E. Mcgee
摘要旁分泌和自分泌生长因子可影响卵泡发育的许多不同方面。转化生长因子β(转化生长因子β)家族的许多成员及其受体在发育中的卵泡中表达。然而，转化生长因子β信号分子家族SMADS的存在和功能在卵泡发育过程中尚未得到评估。我们已经证明两个Smad家族成员同时作为激活素和转化生长因子β的介体，在腔前卵泡的颗粒细胞中表达，但在大的腔卵泡中不表达。在黄体细胞中返回Smad2表达，而不是Smad3表达。转化生长因子β或激活素处理后，Smad2和Smad3均被移位到颗粒细胞的胞核。然而，Smad2对激活素刺激更敏感，Smad3对转化生长因子β刺激更敏感。信号分子的阶段特异性表达和不同的配体敏感性可能共同作用，使转化生长因子β家族配体在卵泡发育过程中使用相同的信号通路产生不同的效果。
Abstract Paracrine and autocrine growth factors can affect many different aspects of ovarian follicle development. Many members of the transforming growth factor β (TGFβ) family of growth factors and their receptors are expressed in developing follicles. However, the presence and function of the family of the TGFβ signaling molecules known as Smads have not been evaluated during follicle development. We have demonstrated that two Smad family members that function as mediators for both activin and TGFβ are expressed in granulosa cells of preantral follicles but not in large antral follicles. Smad2 expression, but not Smad3 expression, returns in luteal cells. Both Smad2 and Smad3 are translocated to the nucleus of granulosa cells in response to treatment with either TGFβ or activin. However, Smad2 is more responsive to activin stimulation, and Smad3 is more responsive to TGFβ stimulation. Stage-specific expression and differing ligand sensitivity of signaling molecules may work together to allow different effects of TGFβ family ligands using the same signaling pathways over the course of follicular development.