Cellular response to horizontally transferred DNA in Escherichia coli is tuned by DNA repair systems
Cellular response to horizontally transferred DNA in Escherichia coli is tuned by DNA repair systems
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DOI：
10.1016/j.dnarep.2004.09.008
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发表时间：
2005-02-03
期刊：
影响因子：
3.8
通讯作者：
Matic, I
中科院分区：
文献类型：
--
作者：
Delmas, S;Matic, I
我们研究了重组DNA和错配修复系统之间的DNA差异如何调节大肠杆菌的SOS反应。SOS诱导和DNA分歧之间观察到的正对数线性相关性，SOS诱导和重组频率之间的负相关性，表明SOS诱导的水平准确地反映了RecA蛋白启动生产性链交换过程的难度。我们的研究结果表明，错配修复系统可以通过影响RecA催化的同源性搜索比通过作用于错配重组中间体来促进SOS诱导。重组机制促进具有最高同一性的序列块之间重组的倾向导致部分二倍体（部分二倍体）相对于真正重组体（同源置换）的比率随着DNA趋异增加而增加。我们讨论了在基因组稳定性和基因组进化的维持中，控制分歧DNA序列之间重组的分子机制的作用。(C)2004 Elsevier B.V.保留所有权利。
We studied how DNA divergence between recombining DNAs and the mismatch repair system modulate the SOS response in Escherichia coli. The observed positive log-linear correlation between SOS induction and DNA divergence, and the negative correlation between SOS induction and frequency of recombination, suggest that the level of SOS induction precisely reflects the difficulty of RecA protein to initiate a productive strand exchange process. Our results suggest that the mismatch repair system could contribute to this SOS induction more by affecting the RecA-catalyzed homology search than by acting on mismatched recombination intermediates. The propensity of the recombination machinery to promote recombination between the blocks of sequences with the highest identity results in the increasing ratios of merodiploids (partial diploids) over genuine recombinants (homologous replacements) with increasing DNA divergence. We discuss the role of molecular mechanisms involved in the control of the recombination between diverged DNA sequences in the maintenance of genomic stability and genome evolution. (C) 2004 Elsevier B.V. All rights reserved.