Evaluation of the efficacy and safety of the Rift Valley Fever Clone 13 vaccine in sheep
Evaluation of the efficacy and safety of the Rift Valley Fever Clone 13 vaccine in sheep
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DOI：
10.1016/j.vaccine.2010.04.085
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发表时间：
2010-06-23
期刊：
影响因子：
5.5
通讯作者：
Bouloy, Michele
中科院分区：
文献类型：
--
作者：
Dungu, Baptiste;Louw, Ian;Bouloy, Michele
在涉及38只处于不同妊娠阶段的母羊、其后代和4只公羊的3个不同实验中，对自然减毒裂谷热（裂谷热）克隆13疫苗在绵羊中的有效性和安全性进行了评价。在实验1中，对4只公羊和总共13只怀孕母羊进行了接种，并在接种期间和用强毒裂谷热病毒攻毒后进行了监测。母羊在妊娠50天或100天接种疫苗，有些在产羔后进行攻毒。在实验2中，9只发情同步化母羊在妊娠50天接种疫苗，并在妊娠100天与5只未接种疫苗的母羊在同一妊娠阶段一起进行攻毒。在实验3中，用3种不同剂量的裂谷热克隆13疫苗接种16只同期发情母羊，并在妊娠30或50天与未接种疫苗的妊娠母羊一起攻毒。三次试验的结果表明，疫苗未引起裂谷热的临床表现，如怀孕母羊流产、后代畸形或所有接种动物发热。接种裂谷热克隆13疫苗还可预防在妊娠不同阶段进行强毒攻击后出现临床裂谷热，而未接种疫苗的对照母羊则出现发热、流产或死于裂谷热。还观察到疫苗剂量反应效应。(C)2010爱思唯尔有限公司版权所有。
The efficacy and safety of the naturally attenuated Rift Valley Fever (RVF) Clone 13 vaccine were evaluated in ovines in three different experiments involving 38 ewes at different stages of pregnancy, their offsprings and four rams. In Experiment 1, 4 rams and a total of 13 pregnant ewes were vaccinated and monitored during vaccination and after a challenge with a virulent RVF virus. The ewes were vaccinated at either 50 or 100 days of pregnancy and some were challenged after lambing. In Experiment 2, nine oestrus-synchronized ewes were vaccinated at 50 days of pregnancy and challenged at 100 days of pregnancy together with 5 unvaccinated ewes at the same stage of pregnancy. In Experiment 3, 16 oestrus-synchronized ewes were vaccinated with 3 different doses of the RVF Clone 13 vaccine and challenged together with unvaccinated pregnant ewes at either 30 or 50 days of pregnancy. The results from the three experiments indicated that the vaccine did not induce clinical manifestation of RVF such as abortion in pregnant ewes, teratogeny in their offsprings, or pyrexia in all vaccinated animals. Vaccination with RVF Clone 13 vaccine also prevented clinical RVF following virulent challenge at different stages of pregnancy while unvaccinated control ewes showed pyrexia, aborted or died of RVF. A vaccine dose-response effect was also observed. (C) 2010 Elsevier Ltd. All rights reserved.