Suppressor of RNA silencing encoded by Beet yellows virus.
Suppressor of RNA silencing encoded by Beet yellows virus.
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甜菜黄化病毒编码的 RNA 沉默抑制剂。
DOI：
10.1016/s0042-6822(02)00051-x
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发表时间：
2003
期刊：
影响因子：
3.7
通讯作者：
Dolja,ValerianV
中科院分区：
文献类型：
--
作者：
Reed,JonathanC;Kasschau,KristinD;Prokhnevsky,AlexeyI;Gopinath,Kodetham;Pogue,GregoryP;Carrington,JamesC;Dolja,ValerianV
使用农杆菌介导的瞬时测定，我们筛选了甜菜黄化病毒的 15.5 kb 基因组，以寻找具有 RNA 沉默抑制活性的蛋白质。在测试的八种蛋白质中，只有 21 kDa 蛋白质 (p21) 能够抑制双链 (ds) RNA 诱导的绿色荧光蛋白 (GFP) mRNA 沉默。在这些条件下，p21 恢复 GFP 表达对小干扰 RNA 的积累没有明显影响。此外，在缺乏 dsRNA 诱导剂的情况下，p21 提高了 GFP mRNA 的瞬时表达水平。使用长线病毒属其他成员编码的 p21 同源物检测到类似的活性。计算机分析表明，p21 样蛋白构成了一个新的蛋白家族，与其他公认的 RNA 沉默抑制因子无关。对 BYV 感染植物中的亚细胞分布的检查表明，p21 在细胞外围分为可溶性细胞质形式和蛋白质包涵体。
Using an Agrobacterium-mediated transient assay, we screened the 15.5-kb genome of the Beet yellows virus for proteins with RNA silencing suppressor activity. Among eight proteins tested, only a 21-kDa protein (p21) was able to suppress double-stranded (ds) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by p21 under these conditions had no apparent effect on accumulation of the small interfering RNAs. In addition, p21 elevated the transient expression level of the GFP mRNA in the absence of dsRNA inducer. Similar activities were detected using homologs of p21 encoded by other members of the genus Closterovirus. Computer analysis indicated that p21-like proteins constitute a novel protein family that is unrelated to other recognized suppressors of RNA silencing. Examination of the subcellular distribution in BYV-infected plants revealed that p21 is partitioned between soluble cytoplasmic form and proteinaceous inclusion bodies at the cell periphery.