Epidemiological characteristics of asymptomatic Norovirus infection in a population from oyster (Ostrea rivularis Gould) farms in southern China
Epidemiological characteristics of asymptomatic Norovirus infection in a population from oyster (Ostrea rivularis Gould) farms in southern China
复制标题
华南地区牡蛎养殖场人群无症状诺如病毒感染流行病学特征
DOI：
10.1017/s0950268818002212
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发表时间：
2018-11-01
影响因子：
4.2
通讯作者：
Zhang, Yonghui
中科院分区：
文献类型：
--
作者：
Wang, Anna;Huang, Qiong;Zhang, Yonghui
摘要本文调查了牡蛎养殖场周围人群无症状诺如病毒感染的流行情况及特点。2014年1月至2014年12月连续两次进行调查，同期共筛选粪便样本4549份。无症状感染者总感染率为4.04%（184/4549）。牡蛎养殖人群诺瓦克病毒感染率为5.20%，显著高于非养殖人群的3.65% （χ2 = 5.49, P < 0.05）。采用实时定量聚合酶链反应（RT-qPCR）和半巢式RT-PCR对184份NoV阳性样品进行鉴定，得到136个序列。这些序列可聚为14个基因型。GI菌株分为GI.2、GI.3、GI.5、GI.6、GI.8和GI.9 6个基因型；GII菌株集中在GII.2、GII.3、GII.4、GII.5、GII.6、GII.8和GII.13。GI.9和gi .17分别是主要和最流行的基因型。2014年，GII.17基因型取代GII.4基因型成为牡蛎养殖区的优势基因型。综上所述，长期监测无症状感染对于发现新的变异菌株和在早期发现疫情至关重要。
Abstract The following paper investigates the prevalence and characteristics of asymptomatic norovirus infection in the population living around oyster farm sites. Two consecutive surveys were conducted from January 2014 to December 2014 and 4549 stool samples were screened during the same time period. The total asymptomatic infection rate was 4.04% (184/4549). Norovirus infection rate was 5.20% in oyster farming population which was significantly higher compared with non-farming population where the infection rate was 3.65% (χ2 = 5.49, P < 0.05). A total of 184 NoV positive samples were identified by real time-quantitative polymerase chain reaction (RT-qPCR) and semi-nested RT-PCR and 136 sequences were obtained. The sequences were clustered into 14 genotypes. GI strains were clustered into six genotypes, including GI.2, GI.3, GI.5, GI.6, GI.8 and GI.9; while GII strains were clustered into GII.2, GII.3, GII.4, GII.5, GII.6, GII.8 and GII.13. GI.9 and GII.17 were the predominant and most prevalent genotypes, respectively. The GII.17 genotype replaced GII.4 becoming the dominant genotype in the oyster farming area in 2014. To sum up, long-term monitoring of asymptomatic infection is crucial for the detection of new variant strains and for identifying outbreaks during the early stage.