Genetic interactions between leaf polarity-controlling genes and ASYMMETRIC LEAVES1 and 2 in Arabidopsis leaf patterning
Genetic interactions between leaf polarity-controlling genes and ASYMMETRIC LEAVES1 and 2 in Arabidopsis leaf patterning
复制标题
拟南芥叶片图案中叶片极性控制基因与 ASYMMETRIC LEAVES1 和 2 之间的遗传相互作用
DOI：
10.1093/pcp/pcm040
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发表时间：
2007-05-01
影响因子：
4.9
通讯作者：
Huang, Hai
中科院分区：
文献类型：
--
作者：
Fu, Yanlei;Xu, Lin;Huang, Hai
在叶片发育过程中，叶片正反极性的建立是叶片正常形态发生的必要条件。这一过程已知受到几个假定的转录因子的严格调控，包括microRNA165/166 （miR165/166）、反式作用短干扰RNA （tasiR-ARF）以及参与RNA沉默的蛋白质。在推测的转录因子基因中，不对称LEA VES1和2 （AS1和2）促进了叶片正面同一性的规范；然而，AS1和AS2与其他叶片极性成分合作的机制仍未确定。在目前的研究中，我们通过将as1和as2与几个关键转录因子的突变结合来表征突变体的表型。我们的数据表明，携带as1/as2和rev、phb或phv的双突变体植株通过产生更严重的倒伏叶片来增强as1/as2缺陷。相比之下，as1/as2与双突变丝状花yabby3 （filyab3）或kanadi1 kanadi2 （kan1 kan2）组合获得的三突变体表现出加性表型。此外，虽然rev as2的叶片中含有高水平的FIL转录本，但miR165/166水平仅略有升高，这表明FIL和miR165/166在叶片模式中平行作用。此外，35S::MIR165a/rev as2转基因植株比35S::MIR165a融合的rev和as2单突变植株产生更严重的倒轴表型。讨论了叶片形成过程中关键调控因子之间的遗传相互作用。
During leaf development, establishment of adaxial-abaxial polarity is essential for normal leaf morphogenesis. This process is known to be strictly regulated by several putative transcription factors, microRNA165/166 (miR165/166), a trans-acting short-interfering RNA (tasiR-ARF), as well as proteins involved in RNA silencing. Among the putative transcription factor genes, ASYMMETRIC LEA VES1 and 2 (AS1 and 2) facilitate the specification of leaf adaxial identity; however, the mechanism by which AS1 and AS2 cooperate with other leaf polarity components remains largely undetermined. In the current study, we characterized the phenotype of mutants by combining as1 and as2 with mutations of several key transcription factors. Our data showed that double mutant plants carrying as1/as2 and rev, phb or phv enhanced as1/as2 defects by producing more severely abaxialized leaves. In contrast, triple mutants, obtained by combining as1/as2 with double mutant filamentous flower yabby3 (fil yab3) or kanadi1 kanadi2 (kan1 kan2), exhibited additive phenotypes. Additionally, while leaves of rev as2 contained high levels of FIL transcripts, only slightly elevated miR165/166 levels were noted, indicating that FIL and miR165/166 act in parallel in leaf patterning. Moreover, 35S::MIR165a/rev as2 transgenic plants resulted in a more severe abaxialized leaf phenotype than the rev and as2 single mutant plants transformed with the same 35S::MIR165a fusion. Genetic interactions between the key regulators during leaf patterning are discussed.