lncRNA Expression after Irradiation and Chemoexposure of HNSCC Cell Lines
lncRNA Expression after Irradiation and Chemoexposure of HNSCC Cell Lines
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DOI：
10.3390/ncrna4040033
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发表时间：
2018-12-01
期刊：
影响因子：
4.3
通讯作者：
Lamperska, Katarzyna
中科院分区：
文献类型：
--
作者：
Guglas, Kacper;Kolenda, Tomasz;Lamperska, Katarzyna
头颈鳞状细胞癌（HNSCC）是世界上第六大最常见的癌症死亡原因。为了提高诊断和患者治疗的质量，需要新的有效的生物标志物。最近的研究表明，不同类型的长非编码 RNA (lncRNA) 的表达水平在 HNSCC 中失调，并与许多生物过程相关。在这项研究中，研究了 HNSCC 细胞系暴露于辐射和细胞毒性药物后 lncRNA 的反应。 SCC-040、SCC-25、FaDu 和 Cal27 细胞系用不同的辐射剂量进行处理，并暴露于顺铂和阿霉素。通过定量逆转录聚合酶链反应（qRT-PCR）检查暴露于这些试剂后lncRNA的表达变化。使用可用的在线工具进行目标预测，并将其分类为特定的生物过程和细胞途径。结果表明，辐射以及化学暴露会导致 lncRNA 表达发生变化，其效果取决于细胞系、药物类型及其剂量。使用 5 Gy 剂量照射后，在所有细胞系中分别观察到 4 个 lncRNA、10 Gy-5 lncRNA 和 20 Gy-3 lncRNA 的显着失调。在所有细胞系中，只有 lncRNA Zfhx2as 下调，与所用剂量无关。暴露于顺铂后，14 个 lncRNA 的表达量降低，只有 2 个 lncRNA 的表达量升高。阿霉素导致 8 种 lncRNA 表达降低，四种 lncRNA 表达增加。在抗 PEG11、BACE1AS、PCGEM1 和 ST7OT 中观察到细胞毒性药物的常见效应。对失调lncRNA预测靶标的分析表明，它们参与重要的生物过程，调节与辐射或化学暴露的直接反应、细胞表型、癌症起始细胞和血管生成相关的细胞途径。辐射和化学暴露都会引起 lncRNA 表达的特定变化。然而，共同效应对于细胞对压力和生存的反应可能很重要。进一步的研究将表明 lncRNA 是否是患者治疗监测的有用工具。
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cause of cancer mortality in the world. To improve the quality of diagnostics and patients' treatment, new and effective biomarkers are needed. Recent studies have shown that the expression level of different types of long non-coding RNAs (lncRNAs) is dysregulated in HNSCC and correlates with many biological processes. In this study, the response of lncRNAs in HNSCC cell lines after exposure to irradiation and cytotoxic drugs was examined. The SCC-040, SCC-25, FaDu, and Cal27 cell lines were treated with different radiation doses as well as exposed to cisplatin and doxorubicin. The expression changes of lncRNAs after exposure to these agents were checked by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Target prediction was performed using available online tools and classified into specific biological processes and cellular pathways. The results indicated that the irradiation, as well as chemoexposure, causes changes in lncRNA expression and the effect depends on the cell line, type of agents as well as their dose. After irradiation using the dose of 5 Gy significant dysregulation of 4 lncRNAs, 10 Gy-5 lncRNAs, and 20 Gy-3 lncRNAs, respectively, were observed in all cell lines. Only lncRNAs Zfhx2as was down-regulated in all cell lines independently of the dose used. After cisplatin exposure, 14 lncRNAs showed lower and only two higher expressions. Doxorubicin resulted in lower expressions of eight and increased four of lncRNAs. Common effects of cytotoxic drugs were observed in the case of antiPEG11, BACE1AS, PCGEM1, and ST7OT. Analysis of the predicted targets for dysregulated lncRNAs indicated that they are involved in important biological processes, regulating cellular pathways connected with direct response to irradiation or chemoexposure, cellular phenotype, cancer initiating cells, and angiogenesis. Both irradiation and chemoexposure caused specific changes in lncRNAs expression. However, the common effect is potentially important for cellular response to the stress and survival. Further study will show if lncRNAs are useful tools in patients' treatment monitoring.