N-linked sugar chain of 55-kDa royal jelly glycoprotein
N-linked sugar chain of 55-kDa royal jelly glycoprotein
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DOI：
10.1271/bbb.60.2099
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发表时间：
1996-12-01
影响因子：
1.6
通讯作者：
Yonekura, M
中科院分区：
文献类型：
--
作者：
Kimura, Y;Kajiyama, S;Yonekura, M
来自55-kDa皇家果冻糖蛋白（RJGP）的N-连接糖链，其维持大鼠肝原代培养细胞的高活力，并且是与350-kDa RJGP不同的分子种类[Kimura et al.，生物科学生物技术生物化学。，59，507-509（1995）]。糖链仅通过肼解，然后N-乙酰化和吡啶胺化而释放。吡啶胺化糖链的结构分析通过连续的内消旋甘露糖苷酶二聚体、MALDI-TOF MS和500 MHz H-1-NMR的组合进行。对于55-kDa RJGP的碳水化合物部分，仅检测到一个N-连接的糖链。已发现该结构为Man alpha 1--> 2 Man alpha 1-->6（Man alpha 1--> 2 Man alpha 1-->3）Man alpha 1-->6（Man alpha 1--> 2 Man alpha 1--> 2 Man alpha 1-->3）Man beta 1--> 4GlcNAc beta 1-->4GlcNAc，其为未加工的高甘露糖型结构。
An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci, Biotech. Biochem., 59, 507-509 (1995)], has been identified, The sugar chains mere released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential eso-mannosidase digestions, MALDI-TOF MS, and 500 MHz H-1-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Man alpha 1-->2Man alpha 1-->6(Man alpha 1-->2Man alpha 1-->3)Man alpha 1-->6(Man alpha 1-->2Man alpha 1-->2Man alpha 1-->3)Man beta 1-->4GlcNAc beta 1-->4GlcNAc, which is a non-processed high mannose type structure.