Scalable robotic biofabrication of tissue spheroids.
Scalable robotic biofabrication of tissue spheroids.
复制标题
组织球体的可扩展机器人生物制造。
DOI：
10.1088/1758-5082/3/2/025002
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发表时间：
2011-06
期刊：
影响因子：
9
通讯作者：
Mironov V
中科院分区：
文献类型：
--
作者：
Mehesz AN;Brown J;Hajdu Z;Beaver W;da Silva JV;Visconti RP;Markwald RR;Mironov V
开发尺寸均匀的组织球体的可扩展生物制造方法对于大尺寸组织和器官构建体的基于组织球体的生物打印至关重要。用于组织球体制造的最新可扩展技术采用在非粘附性水凝胶中制备的微模制凹进模板，其中加载到模板中的细胞由于重力而自组装成组织球体。在这项研究中，我们提出了该技术的改进版本。设计了一种新模具，可以在 96 孔板的每个孔中产生 61 个微凹进。使用 EpMotion 5070 自动移液机将细胞接种到微凹处。孵育 48 小时后，在每个微衰退的底部形成组织球体。为了评估使用该技术生成的构建体的质量，将通过该方法生成的 600 个组织球体与通过传统悬滴法生成的 600 个组织球体进行了比较。这些分析表明，通过微成型方法制造的组织球体直径更均匀。因此，在非粘性水凝胶中使用微模制凹陷，结合自动细胞接种，是可扩展的机器人制造均匀尺寸组织球体的可靠方法。
Development of methods for scalable biofabrication of uniformly sized tissue spheroids is essential for tissue spheroid-based bioprinting of large size tissue and organ constructs. The most recent scalable technique for tissue spheroid fabrication employs a micromolded recessed template prepared in a non-adhesive hydrogel, wherein the cells loaded into the template self-assemble into tissue spheroids due to gravitational force. In this study, we present an improved version of this technique. A new mold was designed to enable generation of 61 microrecessions in each well of a 96-well plate. The microrecessions were seeded with cells using an EpMotion 5070 automated pipetting machine. After 48 h of incubation, tissue spheroids formed at the bottom of each microrecession. To assess the quality of constructs generated using this technology, 600 tissue spheroids made by this method were compared with 600 spheroids generated by the conventional hanging drop method. These analyses showed that tissue spheroids fabricated by the micromolded method are more uniform in diameter. Thus, use of micromolded recessions in a non-adhesive hydrogel, combined with automated cell seeding, is a reliable method for scalable robotic fabrication of uniform-sized tissue spheroids.