Analysis and Purification of Mouse Intestinal Dendritic Cell and Macrophage Subsets by Flow Cytometry.
Analysis and Purification of Mouse Intestinal Dendritic Cell and Macrophage Subsets by Flow Cytometry.
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DOI：
10.1002/cpim.11
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发表时间：
2016-08-01
影响因子：
--
通讯作者：
Bogunovic, Milena
中科院分区：
文献类型：
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作者：
Koscso, Balazs;Bogunovic, Milena
本单位提出了一种用流式细胞术分析小鼠小肠和大肠单细胞悬液中肠道树突状细胞（DC）和巨噬细胞亚群的方法（基本方案）。描述了一种通过Percoll梯度离心来丰富样品中造血细胞部分的策略，并描述了从小肠的特定组织层（如上皮、绒毛粘膜、粘膜下层和外肌层）制备单细胞悬液的方法。最后，支持方案解释了如何通过基于流式细胞术的细胞分选纯化特定的肠DC和巨噬细胞亚群。©2016 by John Wiley & Sons, Inc。
The unit presents a method for analysis of intestinal dendritic cell (DC) and macrophage subsets by flow cytometry in the single cell suspension prepared from the mouse small and large intestine (Basic Protocol). describes a strategy to enrich the hematopoietic cell fraction in the sample by Percoll gradient centrifugation, and describes preparation of single cell suspensions from specific tissue layers of the small intestine, such as the epithelium, villi mucosa, submucosa, and muscularis externa. Finally, Support Protocol explains how to purify specific intestinal DC and macrophage subsets by flow-cytometry-based cell sorting. © 2016 by John Wiley & Sons, Inc.