Importance of oxalate precursors for oxalate metabolism in rats.
Importance of oxalate precursors for oxalate metabolism in rats.
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草酸盐前体对大鼠草酸盐代谢的重要性。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
T. Hatano
中科院分区：
文献类型：
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作者：
Y. Ogawa;T. Miyazato;T. Hatano
用毛细管电泳法测定大鼠尿液中草酸及有关物质的排泄量，比较静脉给药后3种草酸代谢前体的变化。每组8只雄性Wistar大鼠(约200g)每小时采集一次尿样。乙醛酸盐(2毫克)、乙醇酸(10毫克)和羟基丙酮酸(100毫克)根据尿草酸排泄量几乎相同地生成草酸，分别有22.0%、6.1%和0.4%的剂量转化为草酸，3%、8.9%和0.2%转化为乙醇酸，1%、0.1%和0.003%转化为乙醛。草酸平均排泄量在1~2 h达到高峰，乙醇酸在1 h达到高峰，乙醇酸和乙醇酸的基础尿排泄量分别为0.11~0.24和0.0~8.3 nmol/h，3种药物均可引起2~3 h尿乙醇酸排泄显著增加，仅乙醇酸在1 h增加尿乙醛排泄，羟丙酮酸在1~2 h显著增加尿羟丙酮酸、甘油酸和柠檬酸排泄，其原因尚不清楚。
Three metabolic precursors of oxalate were compared after intravenous administration to rats by measuring the urinary excretion of oxalate and related substances using capillary electrophoresis. Urine specimens were collected hourly from eight male Wistar rats (approximately 200 g) in each group. Glyoxylate (2 mg), glycolate (10 mg), and hydroxypyruvate (100 mg) were almost equally oxalogenic based on urinary oxalate excretion, with 22.0, 6.1, and 0.4% of the respective doses being converted into oxalate, 3, 8.9, and 0.2% into glycolate, and 1, 0.1, and 0.003% into glyoxylate. The mean urinary excretion of oxalate peaked between 1 and 2 h, while that of glycolate peaked at 1 h. The baseline urinary excretion of glycolate and glyoxylate was 0.11 to 0.24 micromol/h and 0.0 to 8.3 nmol/h, respectively, and all three agents caused a significant increase of urinary glycolate excretion for 2 to 3 h. Only glyoxylate administration increased urinary glyoxylate excretion at 1 h. Hydroxypyruvate administration significantly increased urinary hydroxypyruvate, glycerate, and citrate excretion at 1 to 2 h. The increase of urinary citrate excretion remains to be explained.