Ureaplasma spp. in male infertility and its relationship with semen quality and seminal plasma components
Ureaplasma spp. in male infertility and its relationship with semen quality and seminal plasma components
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DOI：
10.1016/j.jmii.2016.09.004
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发表时间：
2018-12-01
影响因子：
7.4
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Zhou, Yun Heng;Ma, Hong Xia;Liu, Yang
目的：了解泌尿生殖道支原体感染情况。方法：收集上海地区540例男性不育患者和260例正常生育者精液标本，分别用标准细菌和脲原体（Ureaplasma spp.）文化阳性脲原体属用PCR方法进一步检测脲原体的生物群和血清型。根据世界卫生组织（WHO）人类精液检查和处理实验室手册第五版，采用计算机辅助精液分析技术分析精子形态学变异。结果：尿原体的检出率为100%。不育组和对照组精液标本中阳性率分别为39.6%（214/540）和19.2%（50/260）。两组比较有显著性差异（P < 0.001）。在214例男性不育患者的临床分离株中，59.3%（n = 127）为微小脲原体（UPA），26.2%（n = 56）为解脲脲原体（UUR），14.5%（n = 31）为混合种。对照组（n = 50）分别为64.0%（n = 32）、20.0%（n = 10）、16.0%（n = 8）。两组间差异无统计学意义（P > 0.05）。UPA感染组和混合感染组的前向运动和NAG活性均显著低于UUR感染组（P < 0.05）。在男性不育中起重要致病作用。UPA对附睾前向运动和分泌功能的致病性高于UUR。Copyright（C）2017，Taiwan Society of Microbiology.出版社：Elsevier Taiwan LLC
Objective: We investigated the prevalence of Ureaplasma spp. in semen samples of infertile men in Shanghai, China and evaluated the correlation between the sperm parameters (seminal volume, sperm concentration, progressive motility and non-progressive) and the secretary function in these infectious populations.Methods: Semens were collected from 540 infertile men and 260 fertile control group in shanghai, China and subjected to standard bacterial and Ureaplasma spp. culture. Positive Ureaplasma spp. isolates were further tested by PCR to detect the biovars and serotypes of Ureaplasma spp. Sperm seminological variabilities were analyzed by Computer-Assisted Semen Analysis according to the fifth edition of World Health Organization (WHO) laboratory manual for the examination and processing of human semen. Seminal markers were measured by the automatic analyzer.Results: The prevalence of Ureaplasma spp. in semen specimens was 39.6% (214/540) and 19.2% (50/260) in infertile and control group, respectively. Significant difference was observed between the two groups (P < 0.001). Among all clinical isolates from infertile men (n = 214), 59.3% (n = 127) was Ureaplasma parvum (UPA), 26.2% (n = 56) was Ureaplasma urealyticum (UUR), and 14.5% (n = 31) was mixed species. While those numbers in control group (n = 50) were 64.0% (n = 32), 20.0% (n = 10), 16.0% (n = 8), respectively. There was no significant difference between any two groups (P > 0.05). The progressive motility and the NAG activity of infertile men infected with UPA and mixed species were significantly lower than those of UUR infected subgroup (P < 0.05).Conclusions: The infection of Ureaplasma spp. plays an important pathogenic role in male infertility. UPA has higher pathogenicity on the progressive motility and the secretary function of epididymis than UUR. Copyright (C) 2017, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.