Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering
Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nano-hydroxyapatite/chitosan/chondroitin sulfate/hyaluronic acid for bone tissue engineering
复制标题
用于骨组织工程的基于纳米羟基磷灰石/壳聚糖/硫酸软骨素/透明质酸原位合成的仿生矿化分级混合支架
DOI：
10.1016/j.colsurfb.2017.05.059
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发表时间：
2017-09-01
影响因子：
5.8
通讯作者：
Zhang, Qiqing
中科院分区：
文献类型：
--
作者：
Hu, Yimin;Chen, Jingdi;Zhang, Qiqing
仿生矿化杂化支架是一种模拟细胞外基质（ECM）生物活性的天然骨替代材料，广泛应用于组织工程中。然而，制造具有合适的机械性能和良好的生物相容性的杂化支架仍然是一个挑战。为解决上述问题，本研究成功开发了由纳米羟基磷灰石（nHAP）、壳聚糖（CS）、硫酸软骨素（CSA）和透明质酸（HA）组成的具有分级微/纳米结构的仿生磷酸钙矿化有机-无机杂化支架。在此过程中，一个高效，易于实现的方法相结合的原位仿生合成与冷冻干燥技术。通过傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）对支架的化学结构进行了表征。采用扫描电子显微镜（SEM）对支架的表面形态进行表征。具有均匀分布的孔径的nHAP/CS/CSA/HA杂化支架显示出合适的机械强度，这不仅是由于nHAP的添加，而且是由于带正电荷的CS与带负电荷的CSA和HA之间的相互作用。同时通过MTT细胞毒性试验、碱性磷酸酶（ALP）活性测定、Hoechst 33258荧光染色等方法评价其生物相容性。结果表明，该支架材料具有良好的生物相容性，所添加的成分促进了成骨细胞的增殖和分化。因此，可以预见，原位仿生矿化nHAP/CS/CAS/HA杂化支架将是骨组织工程的有前途的候选者。(C)2017爱思唯尔B. V.保留所有权利。
Biomimetic mineralized hybrid scaffolds are widely used as natural bone substitute materials in tissue engineering by mimicking vital characters of extracellular matrix (ECM). However, the fabrication of hybrid scaffolds with suitable mechanical properties and good biocompatibility remains a challenge. To solve the problems mentioned above, biomimetic calcium phosphate mineralized organic-inorganic hybrid scaffold composed of nano hydroxyapatite (nHAP), Chitosan (CS), Chondroitin sulfate (CSA) and hyaluronic acid (HA) with hierarchical micro/nano structures was successfully developed. In this process, an efficient and easy-to-accomplish method combining in situ biomimetic synthesis with freeze-drying technology was applied. The chemical structure of the scaffolds was confirmed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Surface morphology of scaffolds was characterized by Scanning electron microscopy (SEM). The nHAP/CS/CSA/HA hybrid scaffolds with a well distributed pore size showed suitable mechanical strength which is not only due to the addition of the nHAP but also the interaction between the positively charged CS and the negatively charged CSA and HA. Simultaneously, the biocompatibility was evaluated by the MTT cytotoxicity assay, alkaline phosphatase (ALP) activity, Hoechst 33258 fluorescence staining. All those results proved that the scaffolds possess good biocompatibility and the components added have enhanced the proliferation and differentiation of osteoblast. Thus, it can be anticipated that the in situ biomimetic mineralized nHAP/CS/CAS/HA hybrid scaffolds will be promising candidates for bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.