Isolation of Coronavirus NL63 from Blood from Children in Rural Haiti: Phylogenetic Similarities with Recent Isolates from Malaysia
Isolation of Coronavirus NL63 from Blood from Children in Rural Haiti: Phylogenetic Similarities with Recent Isolates from Malaysia
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DOI：
10.4269/ajtmh.16-0685
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发表时间：
2017-01-01
影响因子：
3.3
通讯作者：
Morris, J. Glenn, Jr.
中科院分区：
文献类型：
--
作者：
De Rochars, Valery Madsen Beau;Lednicky, John;Morris, J. Glenn, Jr.
人类冠状病毒（HCoV）NL 63被认为是上呼吸道感染和流感样疾病的常见原因。在海地农村的一个学校队列中筛查患有急性未分化发热性疾病的儿童时，我们在4名儿童的血液样本中发现了HCoV-NL 63。病例集中在11天内;儿童没有呼吸道症状，但有两个胃肠道投诉。在系统发育分析中，海地HCoV-NL 63菌株聚集在一个高度支持的单系分支中，与最近报道的马来西亚菌株最密切相关;在同一时期在北佛罗里达发现的两种呼吸道HCoV-NL 63菌株形成了一个单独的Glade，尽管再次与马来西亚菌株密切相关。我们的数据突出了HCoV-NL 63可能出现的各种表现，并强调了CoV毒株在国家之间移动的明显容易性，我们的数据与从亚洲向加勒比海地区（海地和佛罗里达）反复引入毒株一致。
Human coronavirus (HCoV) NL63 is recognized as a common cause of upper respiratory infections and influenza-like illness. In screening children with acute undifferentiated febrile illness in a school cohort in rural Haiti, we identified HCoV-NL63 in blood samples from four children. Cases clustered over an 11-day period; children did not have respiratory symptoms, but two had gastrointestinal complaints. On phylogenetic analysis, the Haitian HCoV-NL63 strains cluster together in a highly supported monophyletic clade linked most closely with recently reported strains from Malaysia; two respiratory HCoV-NL63 strains identified in north Florida in the same general period form a separate Glade, albeit again with close linkages with the Malaysian strains. Our data highlight the variety of presentations that may be seen with HCoV-NL63, and underscore the apparent ease with which CoV strains move among countries, with our data consistent with recurrent introduction of strains into the Caribbean (Haiti and Florida) from Asia.