Unique occurrence of pectin-like fibrillar cell wall deposits in xylem fibres of poplar
Unique occurrence of pectin-like fibrillar cell wall deposits in xylem fibres of poplar
复制标题
DOI：
10.1111/j.1438-8677.2008.00082.x
复制
发表时间：
2008-11-01
期刊：
影响因子：
3.9
通讯作者：
Fromm, J.
中科院分区：
文献类型：
--
作者：
Arend, M.;Muninger, M.;Fromm, J.
利用光学显微镜和电子显微镜技术，研究了白杨成熟木质部纤维中纤维细胞壁沉积的独特现象。这些细胞壁沉积物排列在壁的面向管腔的一侧，主要是在靠近血管元件的纤维中。不同的证据表明这些纤维状细胞壁沉积物具有果胶样的性质。首先，通过对果胶物质具有高亲和力的染料Alcian Blue 8GX进行特异性染色。其次，在用果胶酶处理的显微切片中，细胞壁沉积物的染色强烈减少。第三，同时染色的坑，这是已知的主要组成果胶物质。鉴于果胶样性质的纤维状细胞壁沉积物，以及它们的首选发生在纤维附近的水导管元件，纤维状细胞壁沉积物的功能，在横向水扩散和茎水储存的假设。这一假设得到了支持，这些细胞壁沉积物在木材组织中的干旱敏感的白杨品种的丰度增加。
Using light and electron microscopic techniques, we studied the unique occurrence of fibrillar cell wall deposits in mature xylem fibres from poplar. These cell wall deposits lined the lumen-facing side of the wall, mainly in fibres next to vessel elements. Different lines of evidence point to the pectin-like nature of these fibrillar cell wall deposits. First, specific staining by Alcian Blue 8GX, a dye with high affinity for pectic substances. Second, the strongly reduced staining of the cell wall deposits in microscopic sections treated with pectolytic enzyme. Third, concomitant staining of pits, which are known to consist mainly of pectic substances. Given the pectin-like nature of the fibrillar cell wall deposits as well as their preferred occurrence in fibres neighbouring water-conducting vessel elements, a function for the fibrillar cell wall deposits in lateral water diffusion and stem water storage is hypothesised. The hypothesis is supported by the increased abundance of these cell wall deposits in wood tissue of a drought-sensitive poplar species.