High-resolution elemental localization in vacuolate plant cells by nanoscale secondary ion mass spectrometry.
High-resolution elemental localization in vacuolate plant cells by nanoscale secondary ion mass spectrometry.
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DOI：
10.1111/j.1365-313x.2010.04279.x
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发表时间：
2010-09
期刊：
影响因子：
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通讯作者：
K. E. Smart;J. A. Smith;M. Kilburn;Barrie G. H. Martin;C. Hawes;C. Grovenor
中科院分区：
文献类型：
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作者：
K. E. Smart;J. A. Smith;M. Kilburn;Barrie G. H. Martin;C. Hawes;C. Grovenor
通过结合先进的样品制备方法的能力与最新一代的二次离子质谱仪器，我们表明，均匀稀释的物种在生物样品中的分布的化学信息，可以获得优于100 nm的空间分辨率。在这里，我们显示了镍和其他元素的分布在镍超富集植物Alyssum lesbiacum制备的高压冷冻和冷冻置换的叶组织。
By combining the capabilities of advanced sample preparation methodologies with the latest generation of secondary ion mass spectrometry instrumentation, we show that chemical information on the distribution of even dilute species in biological samples can be obtained with spatial resolutions of better than 100 nm. Here, we show the distribution of nickel and other elements in leaf tissue of the nickel hyperaccumulator plant Alyssum lesbiacum prepared by high-pressure freezing and freeze substitution.