First Report of Resistance to Benomyl Fungicide in Sclerotinia sclerotiorum.
First Report of Resistance to Benomyl Fungicide in Sclerotinia sclerotiorum.
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DOI：
10.1094/pdis.2001.85.11.1206c
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发表时间：
2001-11
期刊：
影响因子：
4.5
通讯作者：
B. Gossen;S. Rimmer;J. Holley
中科院分区：
文献类型：
--
作者：
B. Gossen;S. Rimmer;J. Holley
苯菌灵杀菌剂 (Benlate) 在世界范围内用于控制子囊菌病原体，但多个病原体种群已产生抗药性 (1)。在加拿大大草原上，苯菌灵用于减少 Sclerotinia sclerotiorum (Lib.) de Bary 对双低油菜 (Brassica napus, B. rapa) 和苜蓿 (Medicago sativa) 种子作物造成的伤害。为了确定种群是否对苯菌灵具有抗性，将 2000 年从 15 个田地（12 个苜蓿和 3 个油菜，每个田地 1 个分离株）收集的核盘菌分离株培养在用 0、0.05、0.5、5、50 和 500 毫克/升苯菌灵修正的马铃薯葡萄糖琼脂上。将来自活跃生长的菌落边缘的菌丝体置于含有 15 ml 测试培养基的 10 厘米直径培养皿的中心，并在实验室工作台上孵育。 5至6天后测量每个集落（每个集落三个重复）的线性生长（最大宽度和直角的平均值）。低浓度的苯菌灵（EC50 200 毫克/升）抑制了来自 13 个田地的分离株的生长。在研究中仅有的两个怀疑苯菌灵功效降低的田地中，从受感染的双低油菜植物中分离出抗性培养物。该地区耐苯菌灵菌核菌群的分布和重要性仍有待确定。参考文献：(1) T. R. Pettitt 等人。麦考尔。资源。 97：1172，1993 年。
Benomyl fungicide (Benlate) is used worldwide to control ascomycete pathogens, but resistance has developed in several pathogen populations (1). On the Canadian prairies, benomyl is used to reduce injury caused by Sclerotinia sclerotiorum (Lib.) de Bary on canola (Brassica napus, B. rapa) and alfalfa (Medicago sativa) seed crops. To determine if populations are resistant to benomyl, isolates of S. sclerotiorum collected from 15 fields (12 alfalfa and 3 canola, one isolate per field) in 2000 were grown on potato dextrose agar amended with benomyl at 0, 0.05, 0.5, 5, 50, and 500 mg/liter. Plugs of mycelium from the margin of an actively growing colony were placed in the center of a 10-cm-diameter petri dish containing 15 ml of test medium and incubated on a laboratory bench. Linear growth (mean of maximum width and right angle) of each colony (three replicates each) was measured after 5 to 6 days. The growth of isolates from 13 fields was inhibited by low concentrations of benomyl (EC50 200 mg/liter). Resistant cultures were isolated from infected canola plants in the only two fields in the study in which reduced efficacy of benomyl was suspected. The distribution and importance of benomyl-resistant populations of S. sclerotiorum in the region remains to be determined. Reference: (1) T. R. Pettitt et al. Mycol. Res. 97:1172, 1993.